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Non-Invasive Magnetic Resonance Imaging in Rats for Prediction of the Fate of Grafted Kidneys from Cardiac Death Donors
The main objective of this study was to assess cardiac death (CD) kidney grafts before transplantation to determine whether blood oxygen level-dependent (BOLD) and diffusion MRI techniques can predict damage to these grafts after transplantation. We assessed CD kidney tissue by BOLD and diffusion MR...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647057/ https://www.ncbi.nlm.nih.gov/pubmed/23667641 http://dx.doi.org/10.1371/journal.pone.0063573 |
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author | Kaimori, Jun-Ya Iwai, Satomi Hatanaka, Masaki Teratani, Takumi Obi, Yoshitsugu Tsuda, Hidetoshi Isaka, Yoshitaka Yokawa, Takashi Kuroda, Kagayaki Ichimaru, Naotsugu Okumi, Masayoshi Yazawa, Koji Rakugi, Hiromi Nonomura, Norio Takahara, Shiro Kobayashi, Eiji |
author_facet | Kaimori, Jun-Ya Iwai, Satomi Hatanaka, Masaki Teratani, Takumi Obi, Yoshitsugu Tsuda, Hidetoshi Isaka, Yoshitaka Yokawa, Takashi Kuroda, Kagayaki Ichimaru, Naotsugu Okumi, Masayoshi Yazawa, Koji Rakugi, Hiromi Nonomura, Norio Takahara, Shiro Kobayashi, Eiji |
author_sort | Kaimori, Jun-Ya |
collection | PubMed |
description | The main objective of this study was to assess cardiac death (CD) kidney grafts before transplantation to determine whether blood oxygen level-dependent (BOLD) and diffusion MRI techniques can predict damage to these grafts after transplantation. We assessed CD kidney tissue by BOLD and diffusion MRI. We also examined pathological and gene expression changes in CD kidney grafts before and after transplantation. Although there was significantly more red cell congestion (RCC) in the inner stripe of the outer medulla (IS) in both 1 h after cardiac death (CD1h) and CD2h kidneys destined for grafts before transplantation compared with CD0h (p<0.05), CD2h, but not CD1h, kidney grafts had significantly different RCC in the IS 2 days after transplantation (p<0.05). Consistent with these pathological findings, tissue plasminogen activator (tPA) gene expression was increased only in the cortex and medulla of CD2h kidney grafts after transplantation. BOLD MRI successfully and non-invasively imaged and quantified RCC in the IS in both CD1h and CD2h kidney grafts (p<0.05). Diffusion MRI also non-invasively assessed increased the apparent diffusion coefficient in the IS and decreased it in the outer stripe (OS) of CD2h grafts, in concordance with interstitial edema in the IS and tubule cellular edema in the OS. These two types of edema in the outer medulla could explain the prolonged RCC in the IS only of CD2h kidney grafts, creating part of a vicious cycle inhibiting red cells coming out of capillary vessels in the IS. Perfusion with University of Wisconsin solution before MRI measurements did not diminish the difference in tissue damage between CD1h and CD2h kidney grafts. BOLD and diffusion MRI, which are readily available non-invasive tools for evaluating CD kidney grafts tissue damage, can predict prolonged organ damage, and therefore the outcome, of transplanted CD kidney grafts. |
format | Online Article Text |
id | pubmed-3647057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36470572013-05-10 Non-Invasive Magnetic Resonance Imaging in Rats for Prediction of the Fate of Grafted Kidneys from Cardiac Death Donors Kaimori, Jun-Ya Iwai, Satomi Hatanaka, Masaki Teratani, Takumi Obi, Yoshitsugu Tsuda, Hidetoshi Isaka, Yoshitaka Yokawa, Takashi Kuroda, Kagayaki Ichimaru, Naotsugu Okumi, Masayoshi Yazawa, Koji Rakugi, Hiromi Nonomura, Norio Takahara, Shiro Kobayashi, Eiji PLoS One Research Article The main objective of this study was to assess cardiac death (CD) kidney grafts before transplantation to determine whether blood oxygen level-dependent (BOLD) and diffusion MRI techniques can predict damage to these grafts after transplantation. We assessed CD kidney tissue by BOLD and diffusion MRI. We also examined pathological and gene expression changes in CD kidney grafts before and after transplantation. Although there was significantly more red cell congestion (RCC) in the inner stripe of the outer medulla (IS) in both 1 h after cardiac death (CD1h) and CD2h kidneys destined for grafts before transplantation compared with CD0h (p<0.05), CD2h, but not CD1h, kidney grafts had significantly different RCC in the IS 2 days after transplantation (p<0.05). Consistent with these pathological findings, tissue plasminogen activator (tPA) gene expression was increased only in the cortex and medulla of CD2h kidney grafts after transplantation. BOLD MRI successfully and non-invasively imaged and quantified RCC in the IS in both CD1h and CD2h kidney grafts (p<0.05). Diffusion MRI also non-invasively assessed increased the apparent diffusion coefficient in the IS and decreased it in the outer stripe (OS) of CD2h grafts, in concordance with interstitial edema in the IS and tubule cellular edema in the OS. These two types of edema in the outer medulla could explain the prolonged RCC in the IS only of CD2h kidney grafts, creating part of a vicious cycle inhibiting red cells coming out of capillary vessels in the IS. Perfusion with University of Wisconsin solution before MRI measurements did not diminish the difference in tissue damage between CD1h and CD2h kidney grafts. BOLD and diffusion MRI, which are readily available non-invasive tools for evaluating CD kidney grafts tissue damage, can predict prolonged organ damage, and therefore the outcome, of transplanted CD kidney grafts. Public Library of Science 2013-05-07 /pmc/articles/PMC3647057/ /pubmed/23667641 http://dx.doi.org/10.1371/journal.pone.0063573 Text en © 2013 Kaimori et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kaimori, Jun-Ya Iwai, Satomi Hatanaka, Masaki Teratani, Takumi Obi, Yoshitsugu Tsuda, Hidetoshi Isaka, Yoshitaka Yokawa, Takashi Kuroda, Kagayaki Ichimaru, Naotsugu Okumi, Masayoshi Yazawa, Koji Rakugi, Hiromi Nonomura, Norio Takahara, Shiro Kobayashi, Eiji Non-Invasive Magnetic Resonance Imaging in Rats for Prediction of the Fate of Grafted Kidneys from Cardiac Death Donors |
title | Non-Invasive Magnetic Resonance Imaging in Rats for Prediction of the Fate of Grafted Kidneys from Cardiac Death Donors |
title_full | Non-Invasive Magnetic Resonance Imaging in Rats for Prediction of the Fate of Grafted Kidneys from Cardiac Death Donors |
title_fullStr | Non-Invasive Magnetic Resonance Imaging in Rats for Prediction of the Fate of Grafted Kidneys from Cardiac Death Donors |
title_full_unstemmed | Non-Invasive Magnetic Resonance Imaging in Rats for Prediction of the Fate of Grafted Kidneys from Cardiac Death Donors |
title_short | Non-Invasive Magnetic Resonance Imaging in Rats for Prediction of the Fate of Grafted Kidneys from Cardiac Death Donors |
title_sort | non-invasive magnetic resonance imaging in rats for prediction of the fate of grafted kidneys from cardiac death donors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647057/ https://www.ncbi.nlm.nih.gov/pubmed/23667641 http://dx.doi.org/10.1371/journal.pone.0063573 |
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