Highly Efficient NMR Assignment of Intrinsically Disordered Proteins: Application to B- and T Cell Receptor Domains

We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid...

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Detalles Bibliográficos
Autores principales: Isaksson, Linnéa, Mayzel, Maxim, Saline, Maria, Pedersen, Anders, Rosenlöw, Joakim, Brutscher, Bernhard, Karlsson, B. Göran, Orekhov, Vladislav Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647075/
https://www.ncbi.nlm.nih.gov/pubmed/23667548
http://dx.doi.org/10.1371/journal.pone.0062947
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author Isaksson, Linnéa
Mayzel, Maxim
Saline, Maria
Pedersen, Anders
Rosenlöw, Joakim
Brutscher, Bernhard
Karlsson, B. Göran
Orekhov, Vladislav Y.
author_facet Isaksson, Linnéa
Mayzel, Maxim
Saline, Maria
Pedersen, Anders
Rosenlöw, Joakim
Brutscher, Bernhard
Karlsson, B. Göran
Orekhov, Vladislav Y.
author_sort Isaksson, Linnéa
collection PubMed
description We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid automated backbone assignment. The new methodology is applied for characterization of five cytosolic domains from T- and B-cell receptors in solution.
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spelling pubmed-36470752013-05-10 Highly Efficient NMR Assignment of Intrinsically Disordered Proteins: Application to B- and T Cell Receptor Domains Isaksson, Linnéa Mayzel, Maxim Saline, Maria Pedersen, Anders Rosenlöw, Joakim Brutscher, Bernhard Karlsson, B. Göran Orekhov, Vladislav Y. PLoS One Research Article We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid automated backbone assignment. The new methodology is applied for characterization of five cytosolic domains from T- and B-cell receptors in solution. Public Library of Science 2013-05-07 /pmc/articles/PMC3647075/ /pubmed/23667548 http://dx.doi.org/10.1371/journal.pone.0062947 Text en © 2013 Isaksson et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Isaksson, Linnéa
Mayzel, Maxim
Saline, Maria
Pedersen, Anders
Rosenlöw, Joakim
Brutscher, Bernhard
Karlsson, B. Göran
Orekhov, Vladislav Y.
Highly Efficient NMR Assignment of Intrinsically Disordered Proteins: Application to B- and T Cell Receptor Domains
title Highly Efficient NMR Assignment of Intrinsically Disordered Proteins: Application to B- and T Cell Receptor Domains
title_full Highly Efficient NMR Assignment of Intrinsically Disordered Proteins: Application to B- and T Cell Receptor Domains
title_fullStr Highly Efficient NMR Assignment of Intrinsically Disordered Proteins: Application to B- and T Cell Receptor Domains
title_full_unstemmed Highly Efficient NMR Assignment of Intrinsically Disordered Proteins: Application to B- and T Cell Receptor Domains
title_short Highly Efficient NMR Assignment of Intrinsically Disordered Proteins: Application to B- and T Cell Receptor Domains
title_sort highly efficient nmr assignment of intrinsically disordered proteins: application to b- and t cell receptor domains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647075/
https://www.ncbi.nlm.nih.gov/pubmed/23667548
http://dx.doi.org/10.1371/journal.pone.0062947
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