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Highly Efficient NMR Assignment of Intrinsically Disordered Proteins: Application to B- and T Cell Receptor Domains

We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid...

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Detalles Bibliográficos
Autores principales: Isaksson, Linnéa, Mayzel, Maxim, Saline, Maria, Pedersen, Anders, Rosenlöw, Joakim, Brutscher, Bernhard, Karlsson, B. Göran, Orekhov, Vladislav Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647075/
https://www.ncbi.nlm.nih.gov/pubmed/23667548
http://dx.doi.org/10.1371/journal.pone.0062947