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Gene expression profiling of the anti-glioma effect of Cilengitide

Cilengitide (EMD121974), an inhibitor of the adhesive function of integrins, demonstrated preclinical efficacy against malignant glioma. It is speculated that cilengitide can inhibit tumor growth, invasion, and angiogenesis. However, the effects of cilengitide on these processes have not been suffic...

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Autores principales: Onishi, Manabu, Kurozumi, Kazuhiko, Ichikawa, Tomotsugu, Michiue, Hiroyuki, Fujii, Kentaro, Ishida, Joji, Shimazu, Yosuke, Chiocca, E Antonio, Kaur, Balveen, Date, Isao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647089/
https://www.ncbi.nlm.nih.gov/pubmed/23667810
http://dx.doi.org/10.1186/2193-1801-2-160
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author Onishi, Manabu
Kurozumi, Kazuhiko
Ichikawa, Tomotsugu
Michiue, Hiroyuki
Fujii, Kentaro
Ishida, Joji
Shimazu, Yosuke
Chiocca, E Antonio
Kaur, Balveen
Date, Isao
author_facet Onishi, Manabu
Kurozumi, Kazuhiko
Ichikawa, Tomotsugu
Michiue, Hiroyuki
Fujii, Kentaro
Ishida, Joji
Shimazu, Yosuke
Chiocca, E Antonio
Kaur, Balveen
Date, Isao
author_sort Onishi, Manabu
collection PubMed
description Cilengitide (EMD121974), an inhibitor of the adhesive function of integrins, demonstrated preclinical efficacy against malignant glioma. It is speculated that cilengitide can inhibit tumor growth, invasion, and angiogenesis. However, the effects of cilengitide on these processes have not been sufficiently examined. In this study, we investigated the anti-glioma effect of cilengitide using DNA microarray analysis. U87ΔEGFR cells (human malignant glioma cell line) were used for this experiment. The cells were harvested after 16 h of cilengitide treatment, and mRNA was extracted. Gene expression and pathway analyses were performed using a DNA microarray (CodeLink™Human Whole Genome Bioarray). The expression of 265 genes was changed with cilengitide treatment. The expression of 214 genes was up-regulated by more than 4-fold and the expression of 51 genes was down-regulated by more than 4-fold compared to the controls. In pathway analysis, “apoptotic cleavage of cellular proteins” and “TNF receptor signaling pathway” were over-represented. Apoptotic-associated genes such as caspase 8 were up-regulated. Gene expression profiling revealed more detailed mechanism of the anti-glioma effect of cilengitide. Genes associated with apoptosis were over-represented following cilengitide treatment.
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spelling pubmed-36470892013-05-08 Gene expression profiling of the anti-glioma effect of Cilengitide Onishi, Manabu Kurozumi, Kazuhiko Ichikawa, Tomotsugu Michiue, Hiroyuki Fujii, Kentaro Ishida, Joji Shimazu, Yosuke Chiocca, E Antonio Kaur, Balveen Date, Isao Springerplus Research Cilengitide (EMD121974), an inhibitor of the adhesive function of integrins, demonstrated preclinical efficacy against malignant glioma. It is speculated that cilengitide can inhibit tumor growth, invasion, and angiogenesis. However, the effects of cilengitide on these processes have not been sufficiently examined. In this study, we investigated the anti-glioma effect of cilengitide using DNA microarray analysis. U87ΔEGFR cells (human malignant glioma cell line) were used for this experiment. The cells were harvested after 16 h of cilengitide treatment, and mRNA was extracted. Gene expression and pathway analyses were performed using a DNA microarray (CodeLink™Human Whole Genome Bioarray). The expression of 265 genes was changed with cilengitide treatment. The expression of 214 genes was up-regulated by more than 4-fold and the expression of 51 genes was down-regulated by more than 4-fold compared to the controls. In pathway analysis, “apoptotic cleavage of cellular proteins” and “TNF receptor signaling pathway” were over-represented. Apoptotic-associated genes such as caspase 8 were up-regulated. Gene expression profiling revealed more detailed mechanism of the anti-glioma effect of cilengitide. Genes associated with apoptosis were over-represented following cilengitide treatment. Springer International Publishing AG 2013-04-15 /pmc/articles/PMC3647089/ /pubmed/23667810 http://dx.doi.org/10.1186/2193-1801-2-160 Text en © Onishi et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Onishi, Manabu
Kurozumi, Kazuhiko
Ichikawa, Tomotsugu
Michiue, Hiroyuki
Fujii, Kentaro
Ishida, Joji
Shimazu, Yosuke
Chiocca, E Antonio
Kaur, Balveen
Date, Isao
Gene expression profiling of the anti-glioma effect of Cilengitide
title Gene expression profiling of the anti-glioma effect of Cilengitide
title_full Gene expression profiling of the anti-glioma effect of Cilengitide
title_fullStr Gene expression profiling of the anti-glioma effect of Cilengitide
title_full_unstemmed Gene expression profiling of the anti-glioma effect of Cilengitide
title_short Gene expression profiling of the anti-glioma effect of Cilengitide
title_sort gene expression profiling of the anti-glioma effect of cilengitide
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647089/
https://www.ncbi.nlm.nih.gov/pubmed/23667810
http://dx.doi.org/10.1186/2193-1801-2-160
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