Cargando…

No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea

BACKGROUND: Simian virus 40 (SV40), a polyomavirus, was discovered as a contaminant of a human polio vaccine in the 1960s. It is known that malignant mesothelioma (MM) is associated with SV40, and that the virus works as a cofactor to the carcinogenetic effects of asbestos. However, the reports abou...

Descripción completa

Detalles Bibliográficos
Autores principales: Eom, Minseob, Abdul-Ghafar, Jamshid, Park, Sun-Mi, Han, Joung Ho, Hong, Soon Won, Kwon, Kun Young, Ko, Eun Suk, Kim, Lucia, Kim, Wan Seop, Ha, Seung Yeon, Lee, Kyo Young, Lee, Chang Hun, Yoon, Hye Kyoung, Choi, Yoo Duk, Chung, Myoung Ja, Jung, Soon-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Pathologists and The Korean Society for Cytopathology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647124/
https://www.ncbi.nlm.nih.gov/pubmed/23667371
http://dx.doi.org/10.4132/KoreanJPathol.2013.47.2.124
_version_ 1782268692387594240
author Eom, Minseob
Abdul-Ghafar, Jamshid
Park, Sun-Mi
Han, Joung Ho
Hong, Soon Won
Kwon, Kun Young
Ko, Eun Suk
Kim, Lucia
Kim, Wan Seop
Ha, Seung Yeon
Lee, Kyo Young
Lee, Chang Hun
Yoon, Hye Kyoung
Choi, Yoo Duk
Chung, Myoung Ja
Jung, Soon-Hee
author_facet Eom, Minseob
Abdul-Ghafar, Jamshid
Park, Sun-Mi
Han, Joung Ho
Hong, Soon Won
Kwon, Kun Young
Ko, Eun Suk
Kim, Lucia
Kim, Wan Seop
Ha, Seung Yeon
Lee, Kyo Young
Lee, Chang Hun
Yoon, Hye Kyoung
Choi, Yoo Duk
Chung, Myoung Ja
Jung, Soon-Hee
author_sort Eom, Minseob
collection PubMed
description BACKGROUND: Simian virus 40 (SV40), a polyomavirus, was discovered as a contaminant of a human polio vaccine in the 1960s. It is known that malignant mesothelioma (MM) is associated with SV40, and that the virus works as a cofactor to the carcinogenetic effects of asbestos. However, the reports about the correlation between SV40 and MM have not been consistent. The purpose of this study is to identify SV40 in MM tissue in Korea through detection of SV40 protein and DNA. METHODS: We analyzed 62 cases of available paraffin-blocks enrolled through the Korean Malignant Mesothelioma Surveillance System and performed immunohistochemistry for SV40 protein and real-time polymerase chain reaction (PCR) for SV40 DNA. RESULTS: Of 62 total cases, 40 had disease involving the pleura (64.5%), and 29 (46.8%) were found to be of the epithelioid subtype. Immunostaining demonstrated that all examined tissues were negative for SV40 protein. Sufficient DNA was extracted for real-time PCR analysis from 36 cases. Quantitative PCR of these samples showed no increase in SV40 transcript compared to the negative controls. CONCLUSIONS: SV40 is not associated with the development of MM in Korea.
format Online
Article
Text
id pubmed-3647124
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher The Korean Society of Pathologists and The Korean Society for Cytopathology
record_format MEDLINE/PubMed
spelling pubmed-36471242013-05-10 No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea Eom, Minseob Abdul-Ghafar, Jamshid Park, Sun-Mi Han, Joung Ho Hong, Soon Won Kwon, Kun Young Ko, Eun Suk Kim, Lucia Kim, Wan Seop Ha, Seung Yeon Lee, Kyo Young Lee, Chang Hun Yoon, Hye Kyoung Choi, Yoo Duk Chung, Myoung Ja Jung, Soon-Hee Korean J Pathol Original Article BACKGROUND: Simian virus 40 (SV40), a polyomavirus, was discovered as a contaminant of a human polio vaccine in the 1960s. It is known that malignant mesothelioma (MM) is associated with SV40, and that the virus works as a cofactor to the carcinogenetic effects of asbestos. However, the reports about the correlation between SV40 and MM have not been consistent. The purpose of this study is to identify SV40 in MM tissue in Korea through detection of SV40 protein and DNA. METHODS: We analyzed 62 cases of available paraffin-blocks enrolled through the Korean Malignant Mesothelioma Surveillance System and performed immunohistochemistry for SV40 protein and real-time polymerase chain reaction (PCR) for SV40 DNA. RESULTS: Of 62 total cases, 40 had disease involving the pleura (64.5%), and 29 (46.8%) were found to be of the epithelioid subtype. Immunostaining demonstrated that all examined tissues were negative for SV40 protein. Sufficient DNA was extracted for real-time PCR analysis from 36 cases. Quantitative PCR of these samples showed no increase in SV40 transcript compared to the negative controls. CONCLUSIONS: SV40 is not associated with the development of MM in Korea. The Korean Society of Pathologists and The Korean Society for Cytopathology 2013-04 2013-04-24 /pmc/articles/PMC3647124/ /pubmed/23667371 http://dx.doi.org/10.4132/KoreanJPathol.2013.47.2.124 Text en © 2013 The Korean Society of Pathologists/The Korean Society for Cytopathology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Eom, Minseob
Abdul-Ghafar, Jamshid
Park, Sun-Mi
Han, Joung Ho
Hong, Soon Won
Kwon, Kun Young
Ko, Eun Suk
Kim, Lucia
Kim, Wan Seop
Ha, Seung Yeon
Lee, Kyo Young
Lee, Chang Hun
Yoon, Hye Kyoung
Choi, Yoo Duk
Chung, Myoung Ja
Jung, Soon-Hee
No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea
title No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea
title_full No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea
title_fullStr No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea
title_full_unstemmed No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea
title_short No Detection of Simian Virus 40 in Malignant Mesothelioma in Korea
title_sort no detection of simian virus 40 in malignant mesothelioma in korea
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647124/
https://www.ncbi.nlm.nih.gov/pubmed/23667371
http://dx.doi.org/10.4132/KoreanJPathol.2013.47.2.124
work_keys_str_mv AT eomminseob nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT abdulghafarjamshid nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT parksunmi nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT hanjoungho nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT hongsoonwon nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT kwonkunyoung nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT koeunsuk nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT kimlucia nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT kimwanseop nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT haseungyeon nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT leekyoyoung nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT leechanghun nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT yoonhyekyoung nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT choiyooduk nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT chungmyoungja nodetectionofsimianvirus40inmalignantmesotheliomainkorea
AT jungsoonhee nodetectionofsimianvirus40inmalignantmesotheliomainkorea