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SOX7 is down-regulated in lung cancer

BACKGROUND: SOX7 is a transcription factor belonging to the SOX family. Its role in lung cancer is unknown. METHODS: In this study, whole genomic copy number analysis was performed on a series of non-small cell lung cancer (NSCLC) cell lines and samples from individuals with epidermal growth factor...

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Autores principales: Hayano, Takahide, Garg, Manoj, Yin, Dong, Sudo, Makoto, Kawamata, Norihiko, Shi, Shuo, Chien, Wenwen, Ding, Ling-wen, Leong, Geraldine, Mori, Seiichi, Xie, Dong, Tan, Patrick, Koeffler, H Phillip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3648366/
https://www.ncbi.nlm.nih.gov/pubmed/23557216
http://dx.doi.org/10.1186/1756-9966-32-17
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author Hayano, Takahide
Garg, Manoj
Yin, Dong
Sudo, Makoto
Kawamata, Norihiko
Shi, Shuo
Chien, Wenwen
Ding, Ling-wen
Leong, Geraldine
Mori, Seiichi
Xie, Dong
Tan, Patrick
Koeffler, H Phillip
author_facet Hayano, Takahide
Garg, Manoj
Yin, Dong
Sudo, Makoto
Kawamata, Norihiko
Shi, Shuo
Chien, Wenwen
Ding, Ling-wen
Leong, Geraldine
Mori, Seiichi
Xie, Dong
Tan, Patrick
Koeffler, H Phillip
author_sort Hayano, Takahide
collection PubMed
description BACKGROUND: SOX7 is a transcription factor belonging to the SOX family. Its role in lung cancer is unknown. METHODS: In this study, whole genomic copy number analysis was performed on a series of non-small cell lung cancer (NSCLC) cell lines and samples from individuals with epidermal growth factor receptor (EGFR) mutations using a SNP-Chip platform. SOX7 was measured in NSCLC samples and cell lines, and forced expressed in one of these lines. RESULTS: A notable surprise was that the numerous copy number (CN) changes observed in samples of Asian, non-smoking EGFR mutant NSCLC were nearly the same as those CN alterations seen in a large collection of NSCLC from The Cancer Genome Atlas which is presumably composed of predominantly Caucasians who often smoked. However, four regions had CN changes fairly unique to the Asian EGFR mutant group. We also examined CN changes in NSCLC lines. The SOX7 gene was homozygously deleted in one (HCC2935) of 10 NSCLC cell lines and heterozygously deleted in two other NSCLC lines. Expression of SOX7 was significantly downregulated in NSCLC cell lines (8/10, 80%) and a large collection of NSCLC samples compared to matched normal lung (57/62, 92%, p= 0.0006). Forced-expression of SOX7 in NSCLC cell lines markedly reduced their cell growth and enhanced their apoptosis. CONCLUSION: These data suggest that SOX7 is a novel tumor suppressor gene silenced in the majority of NSCLC samples.
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spelling pubmed-36483662013-05-09 SOX7 is down-regulated in lung cancer Hayano, Takahide Garg, Manoj Yin, Dong Sudo, Makoto Kawamata, Norihiko Shi, Shuo Chien, Wenwen Ding, Ling-wen Leong, Geraldine Mori, Seiichi Xie, Dong Tan, Patrick Koeffler, H Phillip J Exp Clin Cancer Res Research BACKGROUND: SOX7 is a transcription factor belonging to the SOX family. Its role in lung cancer is unknown. METHODS: In this study, whole genomic copy number analysis was performed on a series of non-small cell lung cancer (NSCLC) cell lines and samples from individuals with epidermal growth factor receptor (EGFR) mutations using a SNP-Chip platform. SOX7 was measured in NSCLC samples and cell lines, and forced expressed in one of these lines. RESULTS: A notable surprise was that the numerous copy number (CN) changes observed in samples of Asian, non-smoking EGFR mutant NSCLC were nearly the same as those CN alterations seen in a large collection of NSCLC from The Cancer Genome Atlas which is presumably composed of predominantly Caucasians who often smoked. However, four regions had CN changes fairly unique to the Asian EGFR mutant group. We also examined CN changes in NSCLC lines. The SOX7 gene was homozygously deleted in one (HCC2935) of 10 NSCLC cell lines and heterozygously deleted in two other NSCLC lines. Expression of SOX7 was significantly downregulated in NSCLC cell lines (8/10, 80%) and a large collection of NSCLC samples compared to matched normal lung (57/62, 92%, p= 0.0006). Forced-expression of SOX7 in NSCLC cell lines markedly reduced their cell growth and enhanced their apoptosis. CONCLUSION: These data suggest that SOX7 is a novel tumor suppressor gene silenced in the majority of NSCLC samples. BioMed Central 2013-04-04 /pmc/articles/PMC3648366/ /pubmed/23557216 http://dx.doi.org/10.1186/1756-9966-32-17 Text en Copyright © 2013 Hayano et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Hayano, Takahide
Garg, Manoj
Yin, Dong
Sudo, Makoto
Kawamata, Norihiko
Shi, Shuo
Chien, Wenwen
Ding, Ling-wen
Leong, Geraldine
Mori, Seiichi
Xie, Dong
Tan, Patrick
Koeffler, H Phillip
SOX7 is down-regulated in lung cancer
title SOX7 is down-regulated in lung cancer
title_full SOX7 is down-regulated in lung cancer
title_fullStr SOX7 is down-regulated in lung cancer
title_full_unstemmed SOX7 is down-regulated in lung cancer
title_short SOX7 is down-regulated in lung cancer
title_sort sox7 is down-regulated in lung cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3648366/
https://www.ncbi.nlm.nih.gov/pubmed/23557216
http://dx.doi.org/10.1186/1756-9966-32-17
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