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Effect of Oxidative Stress Induced by Brevibacterium sp. BS01 on a HAB Causing Species-Alexandrium tamarense

Harmful algal blooms occur all over the world, destroying aquatic ecosystems and threatening other organisms. The culture supernatant of the marine algicidal actinomycete BS01 was able to lysis dinoflagellate Alexandrium tamarense ATGD98-006. Physiological and biochemical responses to oxidative stres...

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Autores principales: Zhang, Huajun, An, Xinli, Zhou, Yanyan, Zhang, Bangzhou, Zhang, Su, Li, Dong, Chen, Zhangran, Li, Yi, Bai, Shijie, Lv, Jinglin, Zheng, Wei, Tian, Yun, Zheng, Tianling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3648478/
https://www.ncbi.nlm.nih.gov/pubmed/23667564
http://dx.doi.org/10.1371/journal.pone.0063018
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author Zhang, Huajun
An, Xinli
Zhou, Yanyan
Zhang, Bangzhou
Zhang, Su
Li, Dong
Chen, Zhangran
Li, Yi
Bai, Shijie
Lv, Jinglin
Zheng, Wei
Tian, Yun
Zheng, Tianling
author_facet Zhang, Huajun
An, Xinli
Zhou, Yanyan
Zhang, Bangzhou
Zhang, Su
Li, Dong
Chen, Zhangran
Li, Yi
Bai, Shijie
Lv, Jinglin
Zheng, Wei
Tian, Yun
Zheng, Tianling
author_sort Zhang, Huajun
collection PubMed
description Harmful algal blooms occur all over the world, destroying aquatic ecosystems and threatening other organisms. The culture supernatant of the marine algicidal actinomycete BS01 was able to lysis dinoflagellate Alexandrium tamarense ATGD98-006. Physiological and biochemical responses to oxidative stress in A. tamarense were investigated to elucidate the mechanism involved in BS01 inhibition of algal growth. Transmission electron microscope analysis revealed that there were some chloroplast abnormalities in response to BS01 supernatant. The decrease in cellular-soluble protein content suggested that cell growth was greatly inhibited at high concentration of BS01 supernatant. The increase in the levels of reactive oxygen species (ROS) and malondialdehyde contents following exposure to BS01 supernatant indicated that algal cells suffered from oxidative damage. The content of pigment was significantly decreased after 12 h treatment, which indicated that the accumulation of ROS destroyed pigment synthesis. Moreover, the decrease of Fv/Fm ratio suggested that in the photosynthetic system, the dominant sites producing ROS were destroyed by the supernatant of the BS01 culture. The activities of the antioxidant enzymes including superoxide dismutase and peroxidase increased in a short time and decreased slightly with increasing exposure time. A real-time PCR assay showed changes in the transcript abundances of two photosynthetic genes, psbA and psbD. The results showed that BS01 supernatant reduced the expression of the psbA gene after 2 h exposure, but the expression of the psbD gene was increased at concentrations of 1.0 and 1.5%. Our results demonstrated that the expression of the psbA gene was inhibited by the BS01 supernatant, which might block the electron transport chain, significantly enhancing ROS level and excess activity of the antioxidant system. The accumulation of ROS destoryed pigment synthesis and membrane integrity, and inhibited or ultimately killed the algal cells.
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spelling pubmed-36484782013-05-10 Effect of Oxidative Stress Induced by Brevibacterium sp. BS01 on a HAB Causing Species-Alexandrium tamarense Zhang, Huajun An, Xinli Zhou, Yanyan Zhang, Bangzhou Zhang, Su Li, Dong Chen, Zhangran Li, Yi Bai, Shijie Lv, Jinglin Zheng, Wei Tian, Yun Zheng, Tianling PLoS One Research Article Harmful algal blooms occur all over the world, destroying aquatic ecosystems and threatening other organisms. The culture supernatant of the marine algicidal actinomycete BS01 was able to lysis dinoflagellate Alexandrium tamarense ATGD98-006. Physiological and biochemical responses to oxidative stress in A. tamarense were investigated to elucidate the mechanism involved in BS01 inhibition of algal growth. Transmission electron microscope analysis revealed that there were some chloroplast abnormalities in response to BS01 supernatant. The decrease in cellular-soluble protein content suggested that cell growth was greatly inhibited at high concentration of BS01 supernatant. The increase in the levels of reactive oxygen species (ROS) and malondialdehyde contents following exposure to BS01 supernatant indicated that algal cells suffered from oxidative damage. The content of pigment was significantly decreased after 12 h treatment, which indicated that the accumulation of ROS destroyed pigment synthesis. Moreover, the decrease of Fv/Fm ratio suggested that in the photosynthetic system, the dominant sites producing ROS were destroyed by the supernatant of the BS01 culture. The activities of the antioxidant enzymes including superoxide dismutase and peroxidase increased in a short time and decreased slightly with increasing exposure time. A real-time PCR assay showed changes in the transcript abundances of two photosynthetic genes, psbA and psbD. The results showed that BS01 supernatant reduced the expression of the psbA gene after 2 h exposure, but the expression of the psbD gene was increased at concentrations of 1.0 and 1.5%. Our results demonstrated that the expression of the psbA gene was inhibited by the BS01 supernatant, which might block the electron transport chain, significantly enhancing ROS level and excess activity of the antioxidant system. The accumulation of ROS destoryed pigment synthesis and membrane integrity, and inhibited or ultimately killed the algal cells. Public Library of Science 2013-05-08 /pmc/articles/PMC3648478/ /pubmed/23667564 http://dx.doi.org/10.1371/journal.pone.0063018 Text en © 2013 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Huajun
An, Xinli
Zhou, Yanyan
Zhang, Bangzhou
Zhang, Su
Li, Dong
Chen, Zhangran
Li, Yi
Bai, Shijie
Lv, Jinglin
Zheng, Wei
Tian, Yun
Zheng, Tianling
Effect of Oxidative Stress Induced by Brevibacterium sp. BS01 on a HAB Causing Species-Alexandrium tamarense
title Effect of Oxidative Stress Induced by Brevibacterium sp. BS01 on a HAB Causing Species-Alexandrium tamarense
title_full Effect of Oxidative Stress Induced by Brevibacterium sp. BS01 on a HAB Causing Species-Alexandrium tamarense
title_fullStr Effect of Oxidative Stress Induced by Brevibacterium sp. BS01 on a HAB Causing Species-Alexandrium tamarense
title_full_unstemmed Effect of Oxidative Stress Induced by Brevibacterium sp. BS01 on a HAB Causing Species-Alexandrium tamarense
title_short Effect of Oxidative Stress Induced by Brevibacterium sp. BS01 on a HAB Causing Species-Alexandrium tamarense
title_sort effect of oxidative stress induced by brevibacterium sp. bs01 on a hab causing species-alexandrium tamarense
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3648478/
https://www.ncbi.nlm.nih.gov/pubmed/23667564
http://dx.doi.org/10.1371/journal.pone.0063018
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