Bacillus subtilis genome vector-based complete manipulation and reconstruction of genomic DNA for mouse transgenesis

BACKGROUND: The Bacillus subtilis genome (BGM) vector is a novel cloning system for large DNA fragments, in which the entire 4.2 Mb genome of B. subtilis functions as a vector. The BGM vector system has several attractive properties, such as a large cloning capacity of over 3 Mb, stable propagation...

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Detalles Bibliográficos
Autores principales: Iwata, Tetsuo, Kaneko, Shinya, Shiwa, Yuh, Enomoto, Takayuki, Yoshikawa, Hirofumi, Hirota, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3648488/
https://www.ncbi.nlm.nih.gov/pubmed/23642015
http://dx.doi.org/10.1186/1471-2164-14-300

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