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Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle
Intracellular protein trafficking pathways are hijacked by viruses at various stages of viral life-cycle. Heterotetrameric adaptor protein complexes (APs) mediate vesicular trafficking at distinct intracellular sites and are essential for maintaining the organellar homeostasis. In the present study,...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3648799/ https://www.ncbi.nlm.nih.gov/pubmed/23657274 http://dx.doi.org/10.1038/srep01813 |
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author | Agrawal, Tanvi Schu, Peter Medigeshi, Guruprasad R. |
author_facet | Agrawal, Tanvi Schu, Peter Medigeshi, Guruprasad R. |
author_sort | Agrawal, Tanvi |
collection | PubMed |
description | Intracellular protein trafficking pathways are hijacked by viruses at various stages of viral life-cycle. Heterotetrameric adaptor protein complexes (APs) mediate vesicular trafficking at distinct intracellular sites and are essential for maintaining the organellar homeostasis. In the present study, we studied the effect of AP-1 and AP-3 deficiency on flavivirus infection in cells functionally lacking these proteins. We show that AP-1 and AP-3 participate in flavivirus life-cycle at distinct stages. AP-3-deficient cells showed delay in initiation of Japanese encephalitis virus and dengue virus RNA replication, which resulted in reduction of infectious virus production. AP-3 was found to colocalize with RNA replication compartments in infected wild-type cells. AP-1 deficiency affected later stages of dengue virus infection where increased intracellular accumulation of infectious virus was observed. Therefore, our results propose a novel role for AP-1 and AP-3 at distinct stages of infection of some of the RNA viruses. |
format | Online Article Text |
id | pubmed-3648799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-36487992013-05-13 Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle Agrawal, Tanvi Schu, Peter Medigeshi, Guruprasad R. Sci Rep Article Intracellular protein trafficking pathways are hijacked by viruses at various stages of viral life-cycle. Heterotetrameric adaptor protein complexes (APs) mediate vesicular trafficking at distinct intracellular sites and are essential for maintaining the organellar homeostasis. In the present study, we studied the effect of AP-1 and AP-3 deficiency on flavivirus infection in cells functionally lacking these proteins. We show that AP-1 and AP-3 participate in flavivirus life-cycle at distinct stages. AP-3-deficient cells showed delay in initiation of Japanese encephalitis virus and dengue virus RNA replication, which resulted in reduction of infectious virus production. AP-3 was found to colocalize with RNA replication compartments in infected wild-type cells. AP-1 deficiency affected later stages of dengue virus infection where increased intracellular accumulation of infectious virus was observed. Therefore, our results propose a novel role for AP-1 and AP-3 at distinct stages of infection of some of the RNA viruses. Nature Publishing Group 2013-05-09 /pmc/articles/PMC3648799/ /pubmed/23657274 http://dx.doi.org/10.1038/srep01813 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Agrawal, Tanvi Schu, Peter Medigeshi, Guruprasad R. Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle |
title | Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle |
title_full | Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle |
title_fullStr | Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle |
title_full_unstemmed | Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle |
title_short | Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle |
title_sort | adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3648799/ https://www.ncbi.nlm.nih.gov/pubmed/23657274 http://dx.doi.org/10.1038/srep01813 |
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