Cargando…

Endocytic Adaptor Protein Epsin Is Elevated in Prostate Cancer and Required for Cancer Progression

Epsins have an important role in mediating clathrin-mediated endocytosis of ubiquitinated cell surface receptors. The potential role for epsins in tumorigenesis and cancer metastasis by regulating intracellular signaling pathways has largely not been explored. Epsins are reportedly upregulated in se...

Descripción completa

Detalles Bibliográficos
Autores principales: Tessneer, Kandice L., Pasula, Satish, Cai, Xiaofeng, Dong, Yunzhou, Liu, Xiaolei, Yu, Lili, Hahn, Scott, McManus, John, Chen, Yiyuan, Chang, Baojun, Chen, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649151/
https://www.ncbi.nlm.nih.gov/pubmed/23691361
http://dx.doi.org/10.1155/2013/420597
_version_ 1782268912186949632
author Tessneer, Kandice L.
Pasula, Satish
Cai, Xiaofeng
Dong, Yunzhou
Liu, Xiaolei
Yu, Lili
Hahn, Scott
McManus, John
Chen, Yiyuan
Chang, Baojun
Chen, Hong
author_facet Tessneer, Kandice L.
Pasula, Satish
Cai, Xiaofeng
Dong, Yunzhou
Liu, Xiaolei
Yu, Lili
Hahn, Scott
McManus, John
Chen, Yiyuan
Chang, Baojun
Chen, Hong
author_sort Tessneer, Kandice L.
collection PubMed
description Epsins have an important role in mediating clathrin-mediated endocytosis of ubiquitinated cell surface receptors. The potential role for epsins in tumorigenesis and cancer metastasis by regulating intracellular signaling pathways has largely not been explored. Epsins are reportedly upregulated in several types of cancer including human skin, lung, and canine mammary cancers. However, whether their expression is elevated in prostate cancer is unknown. In this study, we investigated the potential role of epsins in prostate tumorigenesis using the wild type or epsin-deficient human prostate cancer cells, LNCaP, in a human xenograft model, and the spontaneous TRAMP mouse model in wild type or epsin-deficient background. Here, we reported that the expression of epsins 1 and 2 is upregulated in both human and mouse prostate cancer cells and cancerous tissues. Consistent with upregulation of epsins in prostate tumors, we discovered that depletion of epsins impaired tumor growth in both the human LNCaP xenograft and the TRAMP mouse prostate. Furthermore, epsin depletion significantly prolonged survival in the TRAMP mouse model. In summary, our findings suggest that epsins may act as oncogenic proteins to promote prostate tumorigenesis and that depletion or inhibition of epsins may provide a novel therapeutic target for future prostate cancer therapies.
format Online
Article
Text
id pubmed-3649151
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-36491512013-05-20 Endocytic Adaptor Protein Epsin Is Elevated in Prostate Cancer and Required for Cancer Progression Tessneer, Kandice L. Pasula, Satish Cai, Xiaofeng Dong, Yunzhou Liu, Xiaolei Yu, Lili Hahn, Scott McManus, John Chen, Yiyuan Chang, Baojun Chen, Hong ISRN Oncol Research Article Epsins have an important role in mediating clathrin-mediated endocytosis of ubiquitinated cell surface receptors. The potential role for epsins in tumorigenesis and cancer metastasis by regulating intracellular signaling pathways has largely not been explored. Epsins are reportedly upregulated in several types of cancer including human skin, lung, and canine mammary cancers. However, whether their expression is elevated in prostate cancer is unknown. In this study, we investigated the potential role of epsins in prostate tumorigenesis using the wild type or epsin-deficient human prostate cancer cells, LNCaP, in a human xenograft model, and the spontaneous TRAMP mouse model in wild type or epsin-deficient background. Here, we reported that the expression of epsins 1 and 2 is upregulated in both human and mouse prostate cancer cells and cancerous tissues. Consistent with upregulation of epsins in prostate tumors, we discovered that depletion of epsins impaired tumor growth in both the human LNCaP xenograft and the TRAMP mouse prostate. Furthermore, epsin depletion significantly prolonged survival in the TRAMP mouse model. In summary, our findings suggest that epsins may act as oncogenic proteins to promote prostate tumorigenesis and that depletion or inhibition of epsins may provide a novel therapeutic target for future prostate cancer therapies. Hindawi Publishing Corporation 2013-04-04 /pmc/articles/PMC3649151/ /pubmed/23691361 http://dx.doi.org/10.1155/2013/420597 Text en Copyright © 2013 Kandice L. Tessneer et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tessneer, Kandice L.
Pasula, Satish
Cai, Xiaofeng
Dong, Yunzhou
Liu, Xiaolei
Yu, Lili
Hahn, Scott
McManus, John
Chen, Yiyuan
Chang, Baojun
Chen, Hong
Endocytic Adaptor Protein Epsin Is Elevated in Prostate Cancer and Required for Cancer Progression
title Endocytic Adaptor Protein Epsin Is Elevated in Prostate Cancer and Required for Cancer Progression
title_full Endocytic Adaptor Protein Epsin Is Elevated in Prostate Cancer and Required for Cancer Progression
title_fullStr Endocytic Adaptor Protein Epsin Is Elevated in Prostate Cancer and Required for Cancer Progression
title_full_unstemmed Endocytic Adaptor Protein Epsin Is Elevated in Prostate Cancer and Required for Cancer Progression
title_short Endocytic Adaptor Protein Epsin Is Elevated in Prostate Cancer and Required for Cancer Progression
title_sort endocytic adaptor protein epsin is elevated in prostate cancer and required for cancer progression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649151/
https://www.ncbi.nlm.nih.gov/pubmed/23691361
http://dx.doi.org/10.1155/2013/420597
work_keys_str_mv AT tessneerkandicel endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression
AT pasulasatish endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression
AT caixiaofeng endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression
AT dongyunzhou endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression
AT liuxiaolei endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression
AT yulili endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression
AT hahnscott endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression
AT mcmanusjohn endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression
AT chenyiyuan endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression
AT changbaojun endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression
AT chenhong endocyticadaptorproteinepsiniselevatedinprostatecancerandrequiredforcancerprogression