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Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection

Foodborne diseases are a major health concern that can have severe impact on society and can add tremendous financial burden to our health care systems. Rapid early detection of food contamination is therefore relevant for the containment of food-borne pathogens. Conventional pathogen detection meth...

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Detalles Bibliográficos
Autores principales: Singh, Amit, Poshtiban, Somayyeh, Evoy, Stephane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649382/
https://www.ncbi.nlm.nih.gov/pubmed/23364199
http://dx.doi.org/10.3390/s130201763
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author Singh, Amit
Poshtiban, Somayyeh
Evoy, Stephane
author_facet Singh, Amit
Poshtiban, Somayyeh
Evoy, Stephane
author_sort Singh, Amit
collection PubMed
description Foodborne diseases are a major health concern that can have severe impact on society and can add tremendous financial burden to our health care systems. Rapid early detection of food contamination is therefore relevant for the containment of food-borne pathogens. Conventional pathogen detection methods, such as microbiological and biochemical identification are time-consuming and laborious, while immunological or nucleic acid-based techniques require extensive sample preparation and are not amenable to miniaturization for on-site detection. Biosensors have shown tremendous promise to overcome these limitations and are being aggressively studied to provide rapid, reliable and sensitive detection platforms for such applications. Novel biological recognition elements are studied to improve the selectivity and facilitate integration on the transduction platform for sensitive detection. Bacteriophages are one such unique biological entity that show excellent host selectivity and have been actively used as recognition probes for pathogen detection. This review summarizes the extensive literature search on the application of bacteriophages (and recently their receptor binding proteins) as probes for sensitive and selective detection of foodborne pathogens, and critically outlines their advantages and disadvantages over other recognition elements.
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spelling pubmed-36493822013-06-04 Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection Singh, Amit Poshtiban, Somayyeh Evoy, Stephane Sensors (Basel) Review Foodborne diseases are a major health concern that can have severe impact on society and can add tremendous financial burden to our health care systems. Rapid early detection of food contamination is therefore relevant for the containment of food-borne pathogens. Conventional pathogen detection methods, such as microbiological and biochemical identification are time-consuming and laborious, while immunological or nucleic acid-based techniques require extensive sample preparation and are not amenable to miniaturization for on-site detection. Biosensors have shown tremendous promise to overcome these limitations and are being aggressively studied to provide rapid, reliable and sensitive detection platforms for such applications. Novel biological recognition elements are studied to improve the selectivity and facilitate integration on the transduction platform for sensitive detection. Bacteriophages are one such unique biological entity that show excellent host selectivity and have been actively used as recognition probes for pathogen detection. This review summarizes the extensive literature search on the application of bacteriophages (and recently their receptor binding proteins) as probes for sensitive and selective detection of foodborne pathogens, and critically outlines their advantages and disadvantages over other recognition elements. Molecular Diversity Preservation International (MDPI) 2013-01-30 /pmc/articles/PMC3649382/ /pubmed/23364199 http://dx.doi.org/10.3390/s130201763 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Singh, Amit
Poshtiban, Somayyeh
Evoy, Stephane
Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection
title Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection
title_full Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection
title_fullStr Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection
title_full_unstemmed Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection
title_short Recent Advances in Bacteriophage Based Biosensors for Food-Borne Pathogen Detection
title_sort recent advances in bacteriophage based biosensors for food-borne pathogen detection
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649382/
https://www.ncbi.nlm.nih.gov/pubmed/23364199
http://dx.doi.org/10.3390/s130201763
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