Cargando…

Interaction Effects of the Leu162Val PPARα and Pro12Ala PPARγ2 Gene Variants with Renal Function in Metabolic Syndrome Population

Leu162Val PPARα and Pro12Ala PPARγ2 were investigated for their individual and their interactive impact on MS and renal functionality (RF). 522 subjects were investigated for biochemical and anthropometric measurements. The diagnosis of MS was based on the IDF definition (2009). The HOMA 2 was used...

Descripción completa

Detalles Bibliográficos
Autores principales: Mohamed Youssef, Sarraj, Mohamed, Najah, Afef, Slimani, Khaldoun, Ben Hamda, Fadoua, Neffati, Fadhel, Najjar Mohamed, Naceur, Slimane Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649708/
https://www.ncbi.nlm.nih.gov/pubmed/23690758
http://dx.doi.org/10.1155/2013/329862
_version_ 1782269022491901952
author Mohamed Youssef, Sarraj
Mohamed, Najah
Afef, Slimani
Khaldoun, Ben Hamda
Fadoua, Neffati
Fadhel, Najjar Mohamed
Naceur, Slimane Mohamed
author_facet Mohamed Youssef, Sarraj
Mohamed, Najah
Afef, Slimani
Khaldoun, Ben Hamda
Fadoua, Neffati
Fadhel, Najjar Mohamed
Naceur, Slimane Mohamed
author_sort Mohamed Youssef, Sarraj
collection PubMed
description Leu162Val PPARα and Pro12Ala PPARγ2 were investigated for their individual and their interactive impact on MS and renal functionality (RF). 522 subjects were investigated for biochemical and anthropometric measurements. The diagnosis of MS was based on the IDF definition (2009). The HOMA 2 was used to determine HOMA-β, HOMA-S and HOMA-IR from FPG and FPI concentrations. RF was assessed by estimating the GFR. PCR-RFLP was performed for DNA genotyping. Allele frequencies were 0.845 for Pro and 0.155 for Ala, and were 0.915 for Leu and 0.085 for Val. We showed that carriers of the PPARα Val 162 allele had lower urea, UA and higher GFR compared to those homozygous for the Leu162 allele. Subjects carried by PPARγ2Ala allele had similar results. They also had reduced FPG, FPI and HOMA-IR, and elevated HOMA-β and HOMA-S compared to those homozygous for the Pro allele. Subjects were divided into 4 groups according to the combinations of genetic alleles of the 2 polymorphisms. Subjects carrying the Leu/Val with an Ala allele had lower FPG, PPI, HOMA-IR, urea, UA levels, higher HOMA-β, HOMA-S and GFR than different genotype combinations. Leu162Val PPARα and Pro12Ala PPARγ2 can interact with each other to modulate glucose and insulin homeostasis and expand their association with overall better RF.
format Online
Article
Text
id pubmed-3649708
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-36497082013-05-20 Interaction Effects of the Leu162Val PPARα and Pro12Ala PPARγ2 Gene Variants with Renal Function in Metabolic Syndrome Population Mohamed Youssef, Sarraj Mohamed, Najah Afef, Slimani Khaldoun, Ben Hamda Fadoua, Neffati Fadhel, Najjar Mohamed Naceur, Slimane Mohamed PPAR Res Research Article Leu162Val PPARα and Pro12Ala PPARγ2 were investigated for their individual and their interactive impact on MS and renal functionality (RF). 522 subjects were investigated for biochemical and anthropometric measurements. The diagnosis of MS was based on the IDF definition (2009). The HOMA 2 was used to determine HOMA-β, HOMA-S and HOMA-IR from FPG and FPI concentrations. RF was assessed by estimating the GFR. PCR-RFLP was performed for DNA genotyping. Allele frequencies were 0.845 for Pro and 0.155 for Ala, and were 0.915 for Leu and 0.085 for Val. We showed that carriers of the PPARα Val 162 allele had lower urea, UA and higher GFR compared to those homozygous for the Leu162 allele. Subjects carried by PPARγ2Ala allele had similar results. They also had reduced FPG, FPI and HOMA-IR, and elevated HOMA-β and HOMA-S compared to those homozygous for the Pro allele. Subjects were divided into 4 groups according to the combinations of genetic alleles of the 2 polymorphisms. Subjects carrying the Leu/Val with an Ala allele had lower FPG, PPI, HOMA-IR, urea, UA levels, higher HOMA-β, HOMA-S and GFR than different genotype combinations. Leu162Val PPARα and Pro12Ala PPARγ2 can interact with each other to modulate glucose and insulin homeostasis and expand their association with overall better RF. Hindawi Publishing Corporation 2013 2013-04-04 /pmc/articles/PMC3649708/ /pubmed/23690758 http://dx.doi.org/10.1155/2013/329862 Text en Copyright © 2013 Sarraj Mohamed Youssef et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mohamed Youssef, Sarraj
Mohamed, Najah
Afef, Slimani
Khaldoun, Ben Hamda
Fadoua, Neffati
Fadhel, Najjar Mohamed
Naceur, Slimane Mohamed
Interaction Effects of the Leu162Val PPARα and Pro12Ala PPARγ2 Gene Variants with Renal Function in Metabolic Syndrome Population
title Interaction Effects of the Leu162Val PPARα and Pro12Ala PPARγ2 Gene Variants with Renal Function in Metabolic Syndrome Population
title_full Interaction Effects of the Leu162Val PPARα and Pro12Ala PPARγ2 Gene Variants with Renal Function in Metabolic Syndrome Population
title_fullStr Interaction Effects of the Leu162Val PPARα and Pro12Ala PPARγ2 Gene Variants with Renal Function in Metabolic Syndrome Population
title_full_unstemmed Interaction Effects of the Leu162Val PPARα and Pro12Ala PPARγ2 Gene Variants with Renal Function in Metabolic Syndrome Population
title_short Interaction Effects of the Leu162Val PPARα and Pro12Ala PPARγ2 Gene Variants with Renal Function in Metabolic Syndrome Population
title_sort interaction effects of the leu162val pparα and pro12ala pparγ2 gene variants with renal function in metabolic syndrome population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649708/
https://www.ncbi.nlm.nih.gov/pubmed/23690758
http://dx.doi.org/10.1155/2013/329862
work_keys_str_mv AT mohamedyoussefsarraj interactioneffectsoftheleu162valpparaandpro12alapparg2genevariantswithrenalfunctioninmetabolicsyndromepopulation
AT mohamednajah interactioneffectsoftheleu162valpparaandpro12alapparg2genevariantswithrenalfunctioninmetabolicsyndromepopulation
AT afefslimani interactioneffectsoftheleu162valpparaandpro12alapparg2genevariantswithrenalfunctioninmetabolicsyndromepopulation
AT khaldounbenhamda interactioneffectsoftheleu162valpparaandpro12alapparg2genevariantswithrenalfunctioninmetabolicsyndromepopulation
AT fadouaneffati interactioneffectsoftheleu162valpparaandpro12alapparg2genevariantswithrenalfunctioninmetabolicsyndromepopulation
AT fadhelnajjarmohamed interactioneffectsoftheleu162valpparaandpro12alapparg2genevariantswithrenalfunctioninmetabolicsyndromepopulation
AT naceurslimanemohamed interactioneffectsoftheleu162valpparaandpro12alapparg2genevariantswithrenalfunctioninmetabolicsyndromepopulation