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Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro

OBJECTIVE: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients. METHODS: The expressi...

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Autores principales: Zhang, Zheng-mao, Yang, Xue-mei, Zhang, Chao, Zhang, Min-jie, Li, Xia, Zhang, Feng-hua, Kang, Shan, Wang, Shi-jie, Shan, Bao-en
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649858/
https://www.ncbi.nlm.nih.gov/pubmed/23671392
http://dx.doi.org/10.2147/OTT.S40504
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author Zhang, Zheng-mao
Yang, Xue-mei
Zhang, Chao
Zhang, Min-jie
Li, Xia
Zhang, Feng-hua
Kang, Shan
Wang, Shi-jie
Shan, Bao-en
author_facet Zhang, Zheng-mao
Yang, Xue-mei
Zhang, Chao
Zhang, Min-jie
Li, Xia
Zhang, Feng-hua
Kang, Shan
Wang, Shi-jie
Shan, Bao-en
author_sort Zhang, Zheng-mao
collection PubMed
description OBJECTIVE: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients. METHODS: The expression levels of interleukin (IL)-10 and transforming growth factor (TGF)-β messenger RNA in peripheral blood were detected by reverse transcription polymerase chain reaction; expression levels of CD80 and CD86 in DCs stimulated by sCD40L were detected using flow cytometry and confocal laser scanning microscopy. RESULTS: Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05). The expression levels of CD80 and CD86 in DCs cultured in the granulocyte-macrophage colony-stimulating factor + IL-4 + stem cell factor + Flt-3 ligand + sCD40L group were significantly increased compared with those in the control group, as assessed by flow cytometry and confocal laser scanning microscopy (P < 0.05). CONCLUSION: A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response.
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spelling pubmed-36498582013-05-13 Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro Zhang, Zheng-mao Yang, Xue-mei Zhang, Chao Zhang, Min-jie Li, Xia Zhang, Feng-hua Kang, Shan Wang, Shi-jie Shan, Bao-en Onco Targets Ther Original Research OBJECTIVE: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients. METHODS: The expression levels of interleukin (IL)-10 and transforming growth factor (TGF)-β messenger RNA in peripheral blood were detected by reverse transcription polymerase chain reaction; expression levels of CD80 and CD86 in DCs stimulated by sCD40L were detected using flow cytometry and confocal laser scanning microscopy. RESULTS: Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05). The expression levels of CD80 and CD86 in DCs cultured in the granulocyte-macrophage colony-stimulating factor + IL-4 + stem cell factor + Flt-3 ligand + sCD40L group were significantly increased compared with those in the control group, as assessed by flow cytometry and confocal laser scanning microscopy (P < 0.05). CONCLUSION: A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response. Dove Medical Press 2013-05-06 /pmc/articles/PMC3649858/ /pubmed/23671392 http://dx.doi.org/10.2147/OTT.S40504 Text en © 2013 Zhang et al, publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Zhang, Zheng-mao
Yang, Xue-mei
Zhang, Chao
Zhang, Min-jie
Li, Xia
Zhang, Feng-hua
Kang, Shan
Wang, Shi-jie
Shan, Bao-en
Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro
title Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro
title_full Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro
title_fullStr Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro
title_full_unstemmed Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro
title_short Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro
title_sort antitumor effects and mechanisms of dendritic cells stimulated by scd40l on ovarian cancer cells in vitro
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649858/
https://www.ncbi.nlm.nih.gov/pubmed/23671392
http://dx.doi.org/10.2147/OTT.S40504
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