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Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro
OBJECTIVE: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients. METHODS: The expressi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649858/ https://www.ncbi.nlm.nih.gov/pubmed/23671392 http://dx.doi.org/10.2147/OTT.S40504 |
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author | Zhang, Zheng-mao Yang, Xue-mei Zhang, Chao Zhang, Min-jie Li, Xia Zhang, Feng-hua Kang, Shan Wang, Shi-jie Shan, Bao-en |
author_facet | Zhang, Zheng-mao Yang, Xue-mei Zhang, Chao Zhang, Min-jie Li, Xia Zhang, Feng-hua Kang, Shan Wang, Shi-jie Shan, Bao-en |
author_sort | Zhang, Zheng-mao |
collection | PubMed |
description | OBJECTIVE: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients. METHODS: The expression levels of interleukin (IL)-10 and transforming growth factor (TGF)-β messenger RNA in peripheral blood were detected by reverse transcription polymerase chain reaction; expression levels of CD80 and CD86 in DCs stimulated by sCD40L were detected using flow cytometry and confocal laser scanning microscopy. RESULTS: Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05). The expression levels of CD80 and CD86 in DCs cultured in the granulocyte-macrophage colony-stimulating factor + IL-4 + stem cell factor + Flt-3 ligand + sCD40L group were significantly increased compared with those in the control group, as assessed by flow cytometry and confocal laser scanning microscopy (P < 0.05). CONCLUSION: A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response. |
format | Online Article Text |
id | pubmed-3649858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-36498582013-05-13 Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro Zhang, Zheng-mao Yang, Xue-mei Zhang, Chao Zhang, Min-jie Li, Xia Zhang, Feng-hua Kang, Shan Wang, Shi-jie Shan, Bao-en Onco Targets Ther Original Research OBJECTIVE: This study aimed to examine the expression of immune suppression factors and the mechanisms of antitumor effects of cord blood dendritic cells (DCs) stimulated by soluble cluster of differentiation 40 ligand (sCD40L) and cytokines in vitro in ovarian cancer patients. METHODS: The expression levels of interleukin (IL)-10 and transforming growth factor (TGF)-β messenger RNA in peripheral blood were detected by reverse transcription polymerase chain reaction; expression levels of CD80 and CD86 in DCs stimulated by sCD40L were detected using flow cytometry and confocal laser scanning microscopy. RESULTS: Expression levels of IL-10 and TGF-β genes in the peripheral blood of ovarian cancer patients were significantly increased compared with patients with benign ovarian tumors (P < 0.05). The expression levels of CD80 and CD86 in DCs cultured in the granulocyte-macrophage colony-stimulating factor + IL-4 + stem cell factor + Flt-3 ligand + sCD40L group were significantly increased compared with those in the control group, as assessed by flow cytometry and confocal laser scanning microscopy (P < 0.05). CONCLUSION: A variety of cytokines in combination with sCD40L can promote the proliferation of cord blood-derived DCs and induce their maturation as well as stimulating a specific antitumor response. Dove Medical Press 2013-05-06 /pmc/articles/PMC3649858/ /pubmed/23671392 http://dx.doi.org/10.2147/OTT.S40504 Text en © 2013 Zhang et al, publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Zhang, Zheng-mao Yang, Xue-mei Zhang, Chao Zhang, Min-jie Li, Xia Zhang, Feng-hua Kang, Shan Wang, Shi-jie Shan, Bao-en Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro |
title | Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro |
title_full | Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro |
title_fullStr | Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro |
title_full_unstemmed | Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro |
title_short | Antitumor effects and mechanisms of dendritic cells stimulated by sCD40L on ovarian cancer cells in vitro |
title_sort | antitumor effects and mechanisms of dendritic cells stimulated by scd40l on ovarian cancer cells in vitro |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649858/ https://www.ncbi.nlm.nih.gov/pubmed/23671392 http://dx.doi.org/10.2147/OTT.S40504 |
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