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Myosin Regulatory Light Chain (RLC) Phosphorylation Change as a Modulator of Cardiac Muscle Contraction in Disease
Understanding how cardiac myosin regulatory light chain (RLC) phosphorylation alters cardiac muscle mechanics is important because it is often altered in cardiac disease. The effect this protein phosphorylation has on muscle mechanics during a physiological range of shortening velocities, during whi...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3650382/ https://www.ncbi.nlm.nih.gov/pubmed/23530050 http://dx.doi.org/10.1074/jbc.M113.455444 |
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author | Toepfer, Christopher Caorsi, Valentina Kampourakis, Thomas Sikkel, Markus B. West, Timothy G. Leung, Man-Ching Al-Saud, Sara A. MacLeod, Kenneth T. Lyon, Alexander R. Marston, Steven B. Sellers, James R. Ferenczi, Michael A. |
author_facet | Toepfer, Christopher Caorsi, Valentina Kampourakis, Thomas Sikkel, Markus B. West, Timothy G. Leung, Man-Ching Al-Saud, Sara A. MacLeod, Kenneth T. Lyon, Alexander R. Marston, Steven B. Sellers, James R. Ferenczi, Michael A. |
author_sort | Toepfer, Christopher |
collection | PubMed |
description | Understanding how cardiac myosin regulatory light chain (RLC) phosphorylation alters cardiac muscle mechanics is important because it is often altered in cardiac disease. The effect this protein phosphorylation has on muscle mechanics during a physiological range of shortening velocities, during which the heart generates power and performs work, has not been addressed. We have expressed and phosphorylated recombinant Rattus norvegicus left ventricular RLC. In vitro we have phosphorylated these recombinant species with cardiac myosin light chain kinase and zipper-interacting protein kinase. We compare rat permeabilized cardiac trabeculae, which have undergone exchange with differently phosphorylated RLC species. We were able to enrich trabecular RLC phosphorylation by 40% compared with controls and, in a separate series, lower RLC phosphorylation to 60% of control values. Compared with the trabeculae with a low level of RLC phosphorylation, RLC phosphorylation enrichment increased isometric force by more than 3-fold and peak power output by more than 7-fold and approximately doubled both maximum shortening speed and the shortening velocity that generated peak power. We augmented these measurements by observing increased RLC phosphorylation of human and rat HF samples from endocardial left ventricular homogenate. These results demonstrate the importance of increased RLC phosphorylation in the up-regulation of myocardial performance and suggest that reduced RLC phosphorylation is a key aspect of impaired contractile function in the diseased myocardium. |
format | Online Article Text |
id | pubmed-3650382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-36503822013-05-14 Myosin Regulatory Light Chain (RLC) Phosphorylation Change as a Modulator of Cardiac Muscle Contraction in Disease Toepfer, Christopher Caorsi, Valentina Kampourakis, Thomas Sikkel, Markus B. West, Timothy G. Leung, Man-Ching Al-Saud, Sara A. MacLeod, Kenneth T. Lyon, Alexander R. Marston, Steven B. Sellers, James R. Ferenczi, Michael A. J Biol Chem Molecular Bases of Disease Understanding how cardiac myosin regulatory light chain (RLC) phosphorylation alters cardiac muscle mechanics is important because it is often altered in cardiac disease. The effect this protein phosphorylation has on muscle mechanics during a physiological range of shortening velocities, during which the heart generates power and performs work, has not been addressed. We have expressed and phosphorylated recombinant Rattus norvegicus left ventricular RLC. In vitro we have phosphorylated these recombinant species with cardiac myosin light chain kinase and zipper-interacting protein kinase. We compare rat permeabilized cardiac trabeculae, which have undergone exchange with differently phosphorylated RLC species. We were able to enrich trabecular RLC phosphorylation by 40% compared with controls and, in a separate series, lower RLC phosphorylation to 60% of control values. Compared with the trabeculae with a low level of RLC phosphorylation, RLC phosphorylation enrichment increased isometric force by more than 3-fold and peak power output by more than 7-fold and approximately doubled both maximum shortening speed and the shortening velocity that generated peak power. We augmented these measurements by observing increased RLC phosphorylation of human and rat HF samples from endocardial left ventricular homogenate. These results demonstrate the importance of increased RLC phosphorylation in the up-regulation of myocardial performance and suggest that reduced RLC phosphorylation is a key aspect of impaired contractile function in the diseased myocardium. American Society for Biochemistry and Molecular Biology 2013-05-10 2013-03-25 /pmc/articles/PMC3650382/ /pubmed/23530050 http://dx.doi.org/10.1074/jbc.M113.455444 Text en © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access. Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/) applies to Author Choice Articles |
spellingShingle | Molecular Bases of Disease Toepfer, Christopher Caorsi, Valentina Kampourakis, Thomas Sikkel, Markus B. West, Timothy G. Leung, Man-Ching Al-Saud, Sara A. MacLeod, Kenneth T. Lyon, Alexander R. Marston, Steven B. Sellers, James R. Ferenczi, Michael A. Myosin Regulatory Light Chain (RLC) Phosphorylation Change as a Modulator of Cardiac Muscle Contraction in Disease |
title | Myosin Regulatory Light Chain (RLC) Phosphorylation Change as a Modulator of Cardiac Muscle Contraction in Disease |
title_full | Myosin Regulatory Light Chain (RLC) Phosphorylation Change as a Modulator of Cardiac Muscle Contraction in Disease |
title_fullStr | Myosin Regulatory Light Chain (RLC) Phosphorylation Change as a Modulator of Cardiac Muscle Contraction in Disease |
title_full_unstemmed | Myosin Regulatory Light Chain (RLC) Phosphorylation Change as a Modulator of Cardiac Muscle Contraction in Disease |
title_short | Myosin Regulatory Light Chain (RLC) Phosphorylation Change as a Modulator of Cardiac Muscle Contraction in Disease |
title_sort | myosin regulatory light chain (rlc) phosphorylation change as a modulator of cardiac muscle contraction in disease |
topic | Molecular Bases of Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3650382/ https://www.ncbi.nlm.nih.gov/pubmed/23530050 http://dx.doi.org/10.1074/jbc.M113.455444 |
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