Genetic screens using the piggyBac transposon

Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strat...

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Detalles Bibliográficos
Autores principales: Chew, Su Kit, Rad, Roland, Futreal, P. Andrew, Bradley, Allan, Liu, Pentao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3650578/
https://www.ncbi.nlm.nih.gov/pubmed/21185377
http://dx.doi.org/10.1016/j.ymeth.2010.12.022
Descripción
Sumario:Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments.

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