Genetic screens using the piggyBac transposon

Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strat...

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Detalles Bibliográficos
Autores principales: Chew, Su Kit, Rad, Roland, Futreal, P. Andrew, Bradley, Allan, Liu, Pentao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3650578/
https://www.ncbi.nlm.nih.gov/pubmed/21185377
http://dx.doi.org/10.1016/j.ymeth.2010.12.022
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author Chew, Su Kit
Rad, Roland
Futreal, P. Andrew
Bradley, Allan
Liu, Pentao
author_facet Chew, Su Kit
Rad, Roland
Futreal, P. Andrew
Bradley, Allan
Liu, Pentao
author_sort Chew, Su Kit
collection PubMed
description Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments.
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spelling pubmed-36505782013-05-13 Genetic screens using the piggyBac transposon Chew, Su Kit Rad, Roland Futreal, P. Andrew Bradley, Allan Liu, Pentao Methods Article Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments. Academic Press 2011-04 /pmc/articles/PMC3650578/ /pubmed/21185377 http://dx.doi.org/10.1016/j.ymeth.2010.12.022 Text en © 2011 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Chew, Su Kit
Rad, Roland
Futreal, P. Andrew
Bradley, Allan
Liu, Pentao
Genetic screens using the piggyBac transposon
title Genetic screens using the piggyBac transposon
title_full Genetic screens using the piggyBac transposon
title_fullStr Genetic screens using the piggyBac transposon
title_full_unstemmed Genetic screens using the piggyBac transposon
title_short Genetic screens using the piggyBac transposon
title_sort genetic screens using the piggybac transposon
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3650578/
https://www.ncbi.nlm.nih.gov/pubmed/21185377
http://dx.doi.org/10.1016/j.ymeth.2010.12.022
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