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The classical progesterone receptor mediates the rapid reduction of fallopian tube ciliary beat frequency by progesterone

BACKGROUND: The transport of gametes as well as the zygote is facilitated by motile cilia lining the inside of the fallopian tube. Progesterone reduces the ciliary beat frequency within 30 minutes in both cows and mice. This rapid reduction suggest the involvement of a non-genomic signaling mechanis...

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Autores principales: Bylander, Anna, Lind, Karin, Goksör, Mattias, Billig, Håkan, Larsson, DG Joakim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651731/
https://www.ncbi.nlm.nih.gov/pubmed/23651709
http://dx.doi.org/10.1186/1477-7827-11-33
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author Bylander, Anna
Lind, Karin
Goksör, Mattias
Billig, Håkan
Larsson, DG Joakim
author_facet Bylander, Anna
Lind, Karin
Goksör, Mattias
Billig, Håkan
Larsson, DG Joakim
author_sort Bylander, Anna
collection PubMed
description BACKGROUND: The transport of gametes as well as the zygote is facilitated by motile cilia lining the inside of the fallopian tube. Progesterone reduces the ciliary beat frequency within 30 minutes in both cows and mice. This rapid reduction suggest the involvement of a non-genomic signaling mechanism, although it is not known which receptors that are involved. Here we investigated the possible involvement of the classical progesterone receptor in this process. METHOD: The ciliary beat frequency of mice fallopian tube was measured ex vivo using an inverted bright field microscope and a high speed camera. The effects of the agonists progesterone and promegestone and an antagonist, mifeprestone, were investigated in wildtype mice. The effect of progesterone was also investigated in mice lacking the classical progesterone receptor. RESULTS: Progesterone, as well as the more specific PR agonist promegestone, significantly reduced the CBF at concentrations of 10–100 nanomolar within 10–30 minutes. In the absence of progesterone, the PR antagonist mifeprestone had no effect on the ciliary beat frequency at a concentration of 1 micromolar. When ciliated cells were pre-incubated with 1 micromolar mifeprestone, addition of progesterone did not reduce the ciliary beat frequency. Accordingly, in ciliated cells from mice not expressing the classical progesterone receptor, exposure to 100 nanomolar progesterone did not reduce the ciliary beat frequency. CONCLUSIONS: This is the first study to provide comprehensive evidence that the classical progesterone receptor mediates the rapid reduction of the tubal ciliary beat frequency by progesterone.
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spelling pubmed-36517312013-05-12 The classical progesterone receptor mediates the rapid reduction of fallopian tube ciliary beat frequency by progesterone Bylander, Anna Lind, Karin Goksör, Mattias Billig, Håkan Larsson, DG Joakim Reprod Biol Endocrinol Research BACKGROUND: The transport of gametes as well as the zygote is facilitated by motile cilia lining the inside of the fallopian tube. Progesterone reduces the ciliary beat frequency within 30 minutes in both cows and mice. This rapid reduction suggest the involvement of a non-genomic signaling mechanism, although it is not known which receptors that are involved. Here we investigated the possible involvement of the classical progesterone receptor in this process. METHOD: The ciliary beat frequency of mice fallopian tube was measured ex vivo using an inverted bright field microscope and a high speed camera. The effects of the agonists progesterone and promegestone and an antagonist, mifeprestone, were investigated in wildtype mice. The effect of progesterone was also investigated in mice lacking the classical progesterone receptor. RESULTS: Progesterone, as well as the more specific PR agonist promegestone, significantly reduced the CBF at concentrations of 10–100 nanomolar within 10–30 minutes. In the absence of progesterone, the PR antagonist mifeprestone had no effect on the ciliary beat frequency at a concentration of 1 micromolar. When ciliated cells were pre-incubated with 1 micromolar mifeprestone, addition of progesterone did not reduce the ciliary beat frequency. Accordingly, in ciliated cells from mice not expressing the classical progesterone receptor, exposure to 100 nanomolar progesterone did not reduce the ciliary beat frequency. CONCLUSIONS: This is the first study to provide comprehensive evidence that the classical progesterone receptor mediates the rapid reduction of the tubal ciliary beat frequency by progesterone. BioMed Central 2013-05-08 /pmc/articles/PMC3651731/ /pubmed/23651709 http://dx.doi.org/10.1186/1477-7827-11-33 Text en Copyright © 2013 Bylander et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Bylander, Anna
Lind, Karin
Goksör, Mattias
Billig, Håkan
Larsson, DG Joakim
The classical progesterone receptor mediates the rapid reduction of fallopian tube ciliary beat frequency by progesterone
title The classical progesterone receptor mediates the rapid reduction of fallopian tube ciliary beat frequency by progesterone
title_full The classical progesterone receptor mediates the rapid reduction of fallopian tube ciliary beat frequency by progesterone
title_fullStr The classical progesterone receptor mediates the rapid reduction of fallopian tube ciliary beat frequency by progesterone
title_full_unstemmed The classical progesterone receptor mediates the rapid reduction of fallopian tube ciliary beat frequency by progesterone
title_short The classical progesterone receptor mediates the rapid reduction of fallopian tube ciliary beat frequency by progesterone
title_sort classical progesterone receptor mediates the rapid reduction of fallopian tube ciliary beat frequency by progesterone
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651731/
https://www.ncbi.nlm.nih.gov/pubmed/23651709
http://dx.doi.org/10.1186/1477-7827-11-33
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