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IL28B SNP screening and distribution in the French Canadian population using a rapid PCR-based test

Single nucleotide polymorphisms (SNPs) in the proximity of the interleukin-28B (IL28B) gene can predict spontaneous resolution of hepatitis C virus (HCV) infection and response to interferon therapy. Screening for this polymorphism has become part of the standard criteria for the management of HCV-i...

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Autores principales: Gélinas, Jean-François, Fabre, Thomas, Willems, Philippe, Leung, Reynold C., George, Jacob, Willems, Bernard, Bruneau, Julie, Shoukry, Naglaa H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651821/
https://www.ncbi.nlm.nih.gov/pubmed/23455512
http://dx.doi.org/10.1007/s00251-013-0688-7
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author Gélinas, Jean-François
Fabre, Thomas
Willems, Philippe
Leung, Reynold C.
George, Jacob
Willems, Bernard
Bruneau, Julie
Shoukry, Naglaa H.
author_facet Gélinas, Jean-François
Fabre, Thomas
Willems, Philippe
Leung, Reynold C.
George, Jacob
Willems, Bernard
Bruneau, Julie
Shoukry, Naglaa H.
author_sort Gélinas, Jean-François
collection PubMed
description Single nucleotide polymorphisms (SNPs) in the proximity of the interleukin-28B (IL28B) gene can predict spontaneous resolution of hepatitis C virus (HCV) infection and response to interferon therapy. Screening for this polymorphism has become part of the standard criteria for the management of HCV-infected patients, hence the need for a rapid, cost-effective screening method. Here, we describe a rapid PCR-based test to screen for two IL28B SNPs (rs12979860 and rs8099917). We used this test to investigate IL28B polymorphism and prevalence in a cohort of French Canadian injection drug users who are part of a unique population known to have a strong genetic founder effect. This population had lower linkage disequilibrium between the two tested SNPs as compared to other cohorts (|d′| = 0.68, r = 0.59). The special genetic makeup should be considered in the management of HCV-infected patients within that population.
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spelling pubmed-36518212013-05-13 IL28B SNP screening and distribution in the French Canadian population using a rapid PCR-based test Gélinas, Jean-François Fabre, Thomas Willems, Philippe Leung, Reynold C. George, Jacob Willems, Bernard Bruneau, Julie Shoukry, Naglaa H. Immunogenetics Original Paper Single nucleotide polymorphisms (SNPs) in the proximity of the interleukin-28B (IL28B) gene can predict spontaneous resolution of hepatitis C virus (HCV) infection and response to interferon therapy. Screening for this polymorphism has become part of the standard criteria for the management of HCV-infected patients, hence the need for a rapid, cost-effective screening method. Here, we describe a rapid PCR-based test to screen for two IL28B SNPs (rs12979860 and rs8099917). We used this test to investigate IL28B polymorphism and prevalence in a cohort of French Canadian injection drug users who are part of a unique population known to have a strong genetic founder effect. This population had lower linkage disequilibrium between the two tested SNPs as compared to other cohorts (|d′| = 0.68, r = 0.59). The special genetic makeup should be considered in the management of HCV-infected patients within that population. Springer-Verlag 2013-03-03 2013 /pmc/articles/PMC3651821/ /pubmed/23455512 http://dx.doi.org/10.1007/s00251-013-0688-7 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Gélinas, Jean-François
Fabre, Thomas
Willems, Philippe
Leung, Reynold C.
George, Jacob
Willems, Bernard
Bruneau, Julie
Shoukry, Naglaa H.
IL28B SNP screening and distribution in the French Canadian population using a rapid PCR-based test
title IL28B SNP screening and distribution in the French Canadian population using a rapid PCR-based test
title_full IL28B SNP screening and distribution in the French Canadian population using a rapid PCR-based test
title_fullStr IL28B SNP screening and distribution in the French Canadian population using a rapid PCR-based test
title_full_unstemmed IL28B SNP screening and distribution in the French Canadian population using a rapid PCR-based test
title_short IL28B SNP screening and distribution in the French Canadian population using a rapid PCR-based test
title_sort il28b snp screening and distribution in the french canadian population using a rapid pcr-based test
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651821/
https://www.ncbi.nlm.nih.gov/pubmed/23455512
http://dx.doi.org/10.1007/s00251-013-0688-7
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