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Role of M2 Muscarinic Receptor in the Airway Response to Methacholine of Mice Selected for Minimal or Maximal Acute Inflammatory Response

Airway smooth muscle constriction induced by cholinergic agonists such as methacholine (MCh), which is typically increased in asthmatic patients, is regulated mainly by muscle muscarinic M3 receptors and negatively by vagal muscarinic M2 receptors. Here we evaluated basal (intrinsic) and allergen-in...

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Autores principales: Castro, Juciane Maria de Andrade, Resende, Rodrigo R., Mirotti, Luciana, Florsheim, Esther, Albuquerque, Layra Lucy, Lino-dos-Santos-Franco, Adriana, Gomes, Eliane, Tavares de Lima, Wothan, de Franco, Marcelo, Ribeiro, Orlando Garcia, Russo, Momtchilo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3652127/
https://www.ncbi.nlm.nih.gov/pubmed/23691511
http://dx.doi.org/10.1155/2013/805627
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author Castro, Juciane Maria de Andrade
Resende, Rodrigo R.
Mirotti, Luciana
Florsheim, Esther
Albuquerque, Layra Lucy
Lino-dos-Santos-Franco, Adriana
Gomes, Eliane
Tavares de Lima, Wothan
de Franco, Marcelo
Ribeiro, Orlando Garcia
Russo, Momtchilo
author_facet Castro, Juciane Maria de Andrade
Resende, Rodrigo R.
Mirotti, Luciana
Florsheim, Esther
Albuquerque, Layra Lucy
Lino-dos-Santos-Franco, Adriana
Gomes, Eliane
Tavares de Lima, Wothan
de Franco, Marcelo
Ribeiro, Orlando Garcia
Russo, Momtchilo
author_sort Castro, Juciane Maria de Andrade
collection PubMed
description Airway smooth muscle constriction induced by cholinergic agonists such as methacholine (MCh), which is typically increased in asthmatic patients, is regulated mainly by muscle muscarinic M3 receptors and negatively by vagal muscarinic M2 receptors. Here we evaluated basal (intrinsic) and allergen-induced (extrinsic) airway responses to MCh. We used two mouse lines selected to respond maximally (AIRmax) or minimally (AIRmin) to innate inflammatory stimuli. We found that in basal condition AIRmin mice responded more vigorously to MCh than AIRmax. Treatment with a specific M2 antagonist increased airway response of AIRmax but not of AIRmin mice. The expression of M2 receptors in the lung was significantly lower in AIRmin compared to AIRmax animals. AIRmax mice developed a more intense allergic inflammation than AIRmin, and both allergic mouse lines increased airway responses to MCh. However, gallamine treatment of allergic groups did not affect the responses to MCh. Our results confirm that low or dysfunctional M2 receptor activity is associated with increased airway responsiveness to MCh and that this trait was inherited during the selective breeding of AIRmin mice and was acquired by AIRmax mice during allergic lung inflammation.
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spelling pubmed-36521272013-05-20 Role of M2 Muscarinic Receptor in the Airway Response to Methacholine of Mice Selected for Minimal or Maximal Acute Inflammatory Response Castro, Juciane Maria de Andrade Resende, Rodrigo R. Mirotti, Luciana Florsheim, Esther Albuquerque, Layra Lucy Lino-dos-Santos-Franco, Adriana Gomes, Eliane Tavares de Lima, Wothan de Franco, Marcelo Ribeiro, Orlando Garcia Russo, Momtchilo Biomed Res Int Research Article Airway smooth muscle constriction induced by cholinergic agonists such as methacholine (MCh), which is typically increased in asthmatic patients, is regulated mainly by muscle muscarinic M3 receptors and negatively by vagal muscarinic M2 receptors. Here we evaluated basal (intrinsic) and allergen-induced (extrinsic) airway responses to MCh. We used two mouse lines selected to respond maximally (AIRmax) or minimally (AIRmin) to innate inflammatory stimuli. We found that in basal condition AIRmin mice responded more vigorously to MCh than AIRmax. Treatment with a specific M2 antagonist increased airway response of AIRmax but not of AIRmin mice. The expression of M2 receptors in the lung was significantly lower in AIRmin compared to AIRmax animals. AIRmax mice developed a more intense allergic inflammation than AIRmin, and both allergic mouse lines increased airway responses to MCh. However, gallamine treatment of allergic groups did not affect the responses to MCh. Our results confirm that low or dysfunctional M2 receptor activity is associated with increased airway responsiveness to MCh and that this trait was inherited during the selective breeding of AIRmin mice and was acquired by AIRmax mice during allergic lung inflammation. Hindawi Publishing Corporation 2013 2013-04-18 /pmc/articles/PMC3652127/ /pubmed/23691511 http://dx.doi.org/10.1155/2013/805627 Text en Copyright © 2013 Juciane Maria de Andrade Castro et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Castro, Juciane Maria de Andrade
Resende, Rodrigo R.
Mirotti, Luciana
Florsheim, Esther
Albuquerque, Layra Lucy
Lino-dos-Santos-Franco, Adriana
Gomes, Eliane
Tavares de Lima, Wothan
de Franco, Marcelo
Ribeiro, Orlando Garcia
Russo, Momtchilo
Role of M2 Muscarinic Receptor in the Airway Response to Methacholine of Mice Selected for Minimal or Maximal Acute Inflammatory Response
title Role of M2 Muscarinic Receptor in the Airway Response to Methacholine of Mice Selected for Minimal or Maximal Acute Inflammatory Response
title_full Role of M2 Muscarinic Receptor in the Airway Response to Methacholine of Mice Selected for Minimal or Maximal Acute Inflammatory Response
title_fullStr Role of M2 Muscarinic Receptor in the Airway Response to Methacholine of Mice Selected for Minimal or Maximal Acute Inflammatory Response
title_full_unstemmed Role of M2 Muscarinic Receptor in the Airway Response to Methacholine of Mice Selected for Minimal or Maximal Acute Inflammatory Response
title_short Role of M2 Muscarinic Receptor in the Airway Response to Methacholine of Mice Selected for Minimal or Maximal Acute Inflammatory Response
title_sort role of m2 muscarinic receptor in the airway response to methacholine of mice selected for minimal or maximal acute inflammatory response
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3652127/
https://www.ncbi.nlm.nih.gov/pubmed/23691511
http://dx.doi.org/10.1155/2013/805627
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