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Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis

We investigated the effect of 1.4% isoflurane (ISO) on the development of inflammation and apoptosis caused by zymosan (ZY) in mice. We found that ZY-challenged mice exhibited significant body weight loss, markedly high mortality, and significant lung injury characterized by the deterioration of his...

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Autores principales: Li, Jun-tang, Wang, Hui, Li, Wei, Wang, Li-feng, Hou, Li-chao, Mu, Jing-lan, Liu, Xin, Chen, Hui-juan, Xie, Ke-lang, Li, Nan-lin, Gao, Chun-fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3652145/
https://www.ncbi.nlm.nih.gov/pubmed/23710113
http://dx.doi.org/10.1155/2013/108928
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author Li, Jun-tang
Wang, Hui
Li, Wei
Wang, Li-feng
Hou, Li-chao
Mu, Jing-lan
Liu, Xin
Chen, Hui-juan
Xie, Ke-lang
Li, Nan-lin
Gao, Chun-fang
author_facet Li, Jun-tang
Wang, Hui
Li, Wei
Wang, Li-feng
Hou, Li-chao
Mu, Jing-lan
Liu, Xin
Chen, Hui-juan
Xie, Ke-lang
Li, Nan-lin
Gao, Chun-fang
author_sort Li, Jun-tang
collection PubMed
description We investigated the effect of 1.4% isoflurane (ISO) on the development of inflammation and apoptosis caused by zymosan (ZY) in mice. We found that ZY-challenged mice exhibited significant body weight loss, markedly high mortality, and significant lung injury characterized by the deterioration of histopathology, histologic scores, and wet-to-dry ratio after ISO treatment. ISO dramatically attenuated ZY-induced lung neutrophil recruitment and inflammation, as evidenced by the reduced levels of total cells, neutrophils, and proinflammatory cytokines (i.e., tumor necrosis factor-α, interleukin- (IL-) 1β, IL-6, and macrophage inflammatory protein-2) in bronchoalveolar lavage fluid and of their mRNA expression in lung tissues. ISO also inhibited ZY-induced expression and activation of nuclear factor-kappaB p65 and inducible nitric oxide synthase in pulmonary tissue. ZY administration also resulted in the upregulation of heme oxygenase-1 expression and activity in the lung, which was further enhanced by ISO treatment. Moreover, ISO markedly prevented ZY-induced pulmonary cell apoptosis in mice, as reflected by the decrease in expression of procaspase-8, procaspase-3, cleaved caspase-8, and cleaved caspase-3, as well as in caspase-3 activity and Bcl-2-associated X/B-cell lymphoma 2 ratio. These results indicate that ISO is a potential therapeutic drug for treating ZY-induced lung injury, and further investigations are warranted.
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spelling pubmed-36521452013-05-24 Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis Li, Jun-tang Wang, Hui Li, Wei Wang, Li-feng Hou, Li-chao Mu, Jing-lan Liu, Xin Chen, Hui-juan Xie, Ke-lang Li, Nan-lin Gao, Chun-fang Mediators Inflamm Research Article We investigated the effect of 1.4% isoflurane (ISO) on the development of inflammation and apoptosis caused by zymosan (ZY) in mice. We found that ZY-challenged mice exhibited significant body weight loss, markedly high mortality, and significant lung injury characterized by the deterioration of histopathology, histologic scores, and wet-to-dry ratio after ISO treatment. ISO dramatically attenuated ZY-induced lung neutrophil recruitment and inflammation, as evidenced by the reduced levels of total cells, neutrophils, and proinflammatory cytokines (i.e., tumor necrosis factor-α, interleukin- (IL-) 1β, IL-6, and macrophage inflammatory protein-2) in bronchoalveolar lavage fluid and of their mRNA expression in lung tissues. ISO also inhibited ZY-induced expression and activation of nuclear factor-kappaB p65 and inducible nitric oxide synthase in pulmonary tissue. ZY administration also resulted in the upregulation of heme oxygenase-1 expression and activity in the lung, which was further enhanced by ISO treatment. Moreover, ISO markedly prevented ZY-induced pulmonary cell apoptosis in mice, as reflected by the decrease in expression of procaspase-8, procaspase-3, cleaved caspase-8, and cleaved caspase-3, as well as in caspase-3 activity and Bcl-2-associated X/B-cell lymphoma 2 ratio. These results indicate that ISO is a potential therapeutic drug for treating ZY-induced lung injury, and further investigations are warranted. Hindawi Publishing Corporation 2013 2013-04-17 /pmc/articles/PMC3652145/ /pubmed/23710113 http://dx.doi.org/10.1155/2013/108928 Text en Copyright © 2013 Jun-tang Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Jun-tang
Wang, Hui
Li, Wei
Wang, Li-feng
Hou, Li-chao
Mu, Jing-lan
Liu, Xin
Chen, Hui-juan
Xie, Ke-lang
Li, Nan-lin
Gao, Chun-fang
Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis
title Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis
title_full Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis
title_fullStr Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis
title_full_unstemmed Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis
title_short Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis
title_sort anesthetic isoflurane posttreatment attenuates experimental lung injury by inhibiting inflammation and apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3652145/
https://www.ncbi.nlm.nih.gov/pubmed/23710113
http://dx.doi.org/10.1155/2013/108928
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