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Distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses

BACKGROUND: Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair. Currently EPCs are defined as either early and late EPCs based on their biological properties and their time of appearance during in vitro culture. EPCs are rare and therefore optimizing isolation...

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Autores principales: Cheng, Cheng-Chung, Chang, Shing-Jyh, Chueh, Yu-Neng, Huang, Tse-Shun, Huang, Po-Hsun, Cheng, Shu-Meng, Tsai, Tsung-Neng, Chen, Jaw-Wen, Wang, Hsei-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3652793/
https://www.ncbi.nlm.nih.gov/pubmed/23496821
http://dx.doi.org/10.1186/1471-2164-14-182
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author Cheng, Cheng-Chung
Chang, Shing-Jyh
Chueh, Yu-Neng
Huang, Tse-Shun
Huang, Po-Hsun
Cheng, Shu-Meng
Tsai, Tsung-Neng
Chen, Jaw-Wen
Wang, Hsei-Wei
author_facet Cheng, Cheng-Chung
Chang, Shing-Jyh
Chueh, Yu-Neng
Huang, Tse-Shun
Huang, Po-Hsun
Cheng, Shu-Meng
Tsai, Tsung-Neng
Chen, Jaw-Wen
Wang, Hsei-Wei
author_sort Cheng, Cheng-Chung
collection PubMed
description BACKGROUND: Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair. Currently EPCs are defined as either early and late EPCs based on their biological properties and their time of appearance during in vitro culture. EPCs are rare and therefore optimizing isolation and culture is required before they can be applied as part of clinical therapies. RESULTS: We compared the gene profiles of early/late EPCs to their ancestors CD133+ or CD34+ stem cells and to matured endothelial cells pinpointing novel biomarkers and stemness genes. Late EPCs were enriched with proliferation and angiogenesis genes, participating in endothelial tubulogenesis and hence neovascularization. Early EPCs expressed abundant inflammatory cytokines and paracrine angiogenic factors, thereby promoting angiogenesis in a paracrine manner. Transcription factors involved in EPC stemness were pinpointed in early EPCs (MAF/MAFB) and in late EPCs (GATA6/IRF6). CONCLUSIONS: The detailed mRNA expression profiles and functional module analysis for different EPCs will help the development of novel therapeutic modalities targeting cardiovascular disease, tumor angiogenesis and various ischemia-related diseases.
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spelling pubmed-36527932013-05-14 Distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses Cheng, Cheng-Chung Chang, Shing-Jyh Chueh, Yu-Neng Huang, Tse-Shun Huang, Po-Hsun Cheng, Shu-Meng Tsai, Tsung-Neng Chen, Jaw-Wen Wang, Hsei-Wei BMC Genomics Research Article BACKGROUND: Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair. Currently EPCs are defined as either early and late EPCs based on their biological properties and their time of appearance during in vitro culture. EPCs are rare and therefore optimizing isolation and culture is required before they can be applied as part of clinical therapies. RESULTS: We compared the gene profiles of early/late EPCs to their ancestors CD133+ or CD34+ stem cells and to matured endothelial cells pinpointing novel biomarkers and stemness genes. Late EPCs were enriched with proliferation and angiogenesis genes, participating in endothelial tubulogenesis and hence neovascularization. Early EPCs expressed abundant inflammatory cytokines and paracrine angiogenic factors, thereby promoting angiogenesis in a paracrine manner. Transcription factors involved in EPC stemness were pinpointed in early EPCs (MAF/MAFB) and in late EPCs (GATA6/IRF6). CONCLUSIONS: The detailed mRNA expression profiles and functional module analysis for different EPCs will help the development of novel therapeutic modalities targeting cardiovascular disease, tumor angiogenesis and various ischemia-related diseases. BioMed Central 2013-03-15 /pmc/articles/PMC3652793/ /pubmed/23496821 http://dx.doi.org/10.1186/1471-2164-14-182 Text en Copyright © 2013 Cheng et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cheng, Cheng-Chung
Chang, Shing-Jyh
Chueh, Yu-Neng
Huang, Tse-Shun
Huang, Po-Hsun
Cheng, Shu-Meng
Tsai, Tsung-Neng
Chen, Jaw-Wen
Wang, Hsei-Wei
Distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses
title Distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses
title_full Distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses
title_fullStr Distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses
title_full_unstemmed Distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses
title_short Distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses
title_sort distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3652793/
https://www.ncbi.nlm.nih.gov/pubmed/23496821
http://dx.doi.org/10.1186/1471-2164-14-182
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