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Diapause Prevention Effect of Bombyx mori by Dimethyl Sulfoxide
HCl treatment has been, for about 80 years, the primary method for the prevention of entry into embryonic diapauses of Bombyx mori. This is because no method is as effective as the HCl treatment. In this study, we discovered that dimethyl sulfoxide (DMSO) prevented entry into the diapause of the sil...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3652847/ https://www.ncbi.nlm.nih.gov/pubmed/23675522 http://dx.doi.org/10.1371/journal.pone.0064124 |
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author | Yamamoto, Takayuki Mase, Keisuke Sawada, Hiroshi |
author_facet | Yamamoto, Takayuki Mase, Keisuke Sawada, Hiroshi |
author_sort | Yamamoto, Takayuki |
collection | PubMed |
description | HCl treatment has been, for about 80 years, the primary method for the prevention of entry into embryonic diapauses of Bombyx mori. This is because no method is as effective as the HCl treatment. In this study, we discovered that dimethyl sulfoxide (DMSO) prevented entry into the diapause of the silkworm, Bombyx mori. The effect of diapause prevention was 78% as a result of treatment with 100% DMSO concentration, and the effect was comparable to that of the HCl treatment. In contrast, in the case of non-diapause eggs, hatchability was decreased by DMSO in a concentration-dependent manner. The effect of DMSO was restricted within 24 hours after oviposition of diapause eggs, and the critical period was slightly shorter than the effective period of the HCl treatment. DMSO analogs, such as dimethyl formamide (DMF) and dimethyl sulfide (DMS), did little preventive effect against the diapause. Furthermore, we also investigated the permeation effects of chemical compounds by DMSO. When treated with an inhibitor of protein kinase CK2 (CK2) dissolved in DMSO, the prevention rate of the diapause was less than 40%. This means that the inhibition effect by the CK2 inhibitor was the inhibition of embryonic development after diapause prevention by DMSO. These data suggest that DMSO has the effects of preventing from entering into the diapause and permeation of chemicals into diapause eggs. |
format | Online Article Text |
id | pubmed-3652847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36528472013-05-14 Diapause Prevention Effect of Bombyx mori by Dimethyl Sulfoxide Yamamoto, Takayuki Mase, Keisuke Sawada, Hiroshi PLoS One Research Article HCl treatment has been, for about 80 years, the primary method for the prevention of entry into embryonic diapauses of Bombyx mori. This is because no method is as effective as the HCl treatment. In this study, we discovered that dimethyl sulfoxide (DMSO) prevented entry into the diapause of the silkworm, Bombyx mori. The effect of diapause prevention was 78% as a result of treatment with 100% DMSO concentration, and the effect was comparable to that of the HCl treatment. In contrast, in the case of non-diapause eggs, hatchability was decreased by DMSO in a concentration-dependent manner. The effect of DMSO was restricted within 24 hours after oviposition of diapause eggs, and the critical period was slightly shorter than the effective period of the HCl treatment. DMSO analogs, such as dimethyl formamide (DMF) and dimethyl sulfide (DMS), did little preventive effect against the diapause. Furthermore, we also investigated the permeation effects of chemical compounds by DMSO. When treated with an inhibitor of protein kinase CK2 (CK2) dissolved in DMSO, the prevention rate of the diapause was less than 40%. This means that the inhibition effect by the CK2 inhibitor was the inhibition of embryonic development after diapause prevention by DMSO. These data suggest that DMSO has the effects of preventing from entering into the diapause and permeation of chemicals into diapause eggs. Public Library of Science 2013-05-13 /pmc/articles/PMC3652847/ /pubmed/23675522 http://dx.doi.org/10.1371/journal.pone.0064124 Text en © 2013 Yamamoto et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Yamamoto, Takayuki Mase, Keisuke Sawada, Hiroshi Diapause Prevention Effect of Bombyx mori by Dimethyl Sulfoxide |
title | Diapause Prevention Effect of Bombyx mori by Dimethyl Sulfoxide |
title_full | Diapause Prevention Effect of Bombyx mori by Dimethyl Sulfoxide |
title_fullStr | Diapause Prevention Effect of Bombyx mori by Dimethyl Sulfoxide |
title_full_unstemmed | Diapause Prevention Effect of Bombyx mori by Dimethyl Sulfoxide |
title_short | Diapause Prevention Effect of Bombyx mori by Dimethyl Sulfoxide |
title_sort | diapause prevention effect of bombyx mori by dimethyl sulfoxide |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3652847/ https://www.ncbi.nlm.nih.gov/pubmed/23675522 http://dx.doi.org/10.1371/journal.pone.0064124 |
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