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LSSP-PCR of Trypanosoma cruzi: how the single primer sequence affects the kDNA signature

BACKGROUND: Low-stringency single specific primer PCR (LSSP-PCR) is a highly sensitive and discriminating technique that has been extensively used to genetically characterize Trypanosoma cruzi populations in the presence of large amounts of host DNA. To ensure high sensitivity, in most T. cruzi stud...

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Detalles Bibliográficos
Autores principales: Segatto, Marcela, Rodrigues, Claudiney Melquíades, Machado, Carlos Renato, Franco, Glória Regina, Pena, Sérgio Danilo Junho, Macedo, Andréa Mara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653686/
https://www.ncbi.nlm.nih.gov/pubmed/23639061
http://dx.doi.org/10.1186/1756-0500-6-174

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