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Effects of smoking and smoking cessation on human serum metabolite profile: results from the KORA cohort study
BACKGROUND: Metabolomics helps to identify links between environmental exposures and intermediate biomarkers of disturbed pathways. We previously reported variations in phosphatidylcholines in male smokers compared with non-smokers in a cross-sectional pilot study with a small sample size, but knowl...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653729/ https://www.ncbi.nlm.nih.gov/pubmed/23497222 http://dx.doi.org/10.1186/1741-7015-11-60 |
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author | Xu, Tao Holzapfel, Christina Dong, Xiao Bader, Erik Yu, Zhonghao Prehn, Cornelia Perstorfer, Katrin Jaremek, Marta Roemisch-Margl, Werner Rathmann, Wolfgang Li, Yixue Wichmann, H -Erich Wallaschofski, Henri Ladwig, Karl H Theis, Fabian Suhre, Karsten Adamski, Jerzy Illig, Thomas Peters, Annette Wang-Sattler, Rui |
author_facet | Xu, Tao Holzapfel, Christina Dong, Xiao Bader, Erik Yu, Zhonghao Prehn, Cornelia Perstorfer, Katrin Jaremek, Marta Roemisch-Margl, Werner Rathmann, Wolfgang Li, Yixue Wichmann, H -Erich Wallaschofski, Henri Ladwig, Karl H Theis, Fabian Suhre, Karsten Adamski, Jerzy Illig, Thomas Peters, Annette Wang-Sattler, Rui |
author_sort | Xu, Tao |
collection | PubMed |
description | BACKGROUND: Metabolomics helps to identify links between environmental exposures and intermediate biomarkers of disturbed pathways. We previously reported variations in phosphatidylcholines in male smokers compared with non-smokers in a cross-sectional pilot study with a small sample size, but knowledge of the reversibility of smoking effects on metabolite profiles is limited. Here, we extend our metabolomics study with a large prospective study including female smokers and quitters. METHODS: Using targeted metabolomics approach, we quantified 140 metabolite concentrations for 1,241 fasting serum samples in the population-based Cooperative Health Research in the Region of Augsburg (KORA) human cohort at two time points: baseline survey conducted between 1999 and 2001 and follow-up after seven years. Metabolite profiles were compared among groups of current smokers, former smokers and never smokers, and were further assessed for their reversibility after smoking cessation. Changes in metabolite concentrations from baseline to the follow-up were investigated in a longitudinal analysis comparing current smokers, never smokers and smoking quitters, who were current smokers at baseline but former smokers by the time of follow-up. In addition, we constructed protein-metabolite networks with smoking-related genes and metabolites. RESULTS: We identified 21 smoking-related metabolites in the baseline investigation (18 in men and six in women, with three overlaps) enriched in amino acid and lipid pathways, which were significantly different between current smokers and never smokers. Moreover, 19 out of the 21 metabolites were found to be reversible in former smokers. In the follow-up study, 13 reversible metabolites in men were measured, of which 10 were confirmed to be reversible in male quitters. Protein-metabolite networks are proposed to explain the consistent reversibility of smoking effects on metabolites. CONCLUSIONS: We showed that smoking-related changes in human serum metabolites are reversible after smoking cessation, consistent with the known cardiovascular risk reduction. The metabolites identified may serve as potential biomarkers to evaluate the status of smoking cessation and characterize smoking-related diseases. |
format | Online Article Text |
id | pubmed-3653729 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36537292013-05-16 Effects of smoking and smoking cessation on human serum metabolite profile: results from the KORA cohort study Xu, Tao Holzapfel, Christina Dong, Xiao Bader, Erik Yu, Zhonghao Prehn, Cornelia Perstorfer, Katrin Jaremek, Marta Roemisch-Margl, Werner Rathmann, Wolfgang Li, Yixue Wichmann, H -Erich Wallaschofski, Henri Ladwig, Karl H Theis, Fabian Suhre, Karsten Adamski, Jerzy Illig, Thomas Peters, Annette Wang-Sattler, Rui BMC Med Research Article BACKGROUND: Metabolomics helps to identify links between environmental exposures and intermediate biomarkers of disturbed pathways. We previously reported variations in phosphatidylcholines in male smokers compared with non-smokers in a cross-sectional pilot study with a small sample size, but knowledge of the reversibility of smoking effects on metabolite profiles is limited. Here, we extend our metabolomics study with a large prospective study including female smokers and quitters. METHODS: Using targeted metabolomics approach, we quantified 140 metabolite concentrations for 1,241 fasting serum samples in the population-based Cooperative Health Research in the Region of Augsburg (KORA) human cohort at two time points: baseline survey conducted between 1999 and 2001 and follow-up after seven years. Metabolite profiles were compared among groups of current smokers, former smokers and never smokers, and were further assessed for their reversibility after smoking cessation. Changes in metabolite concentrations from baseline to the follow-up were investigated in a longitudinal analysis comparing current smokers, never smokers and smoking quitters, who were current smokers at baseline but former smokers by the time of follow-up. In addition, we constructed protein-metabolite networks with smoking-related genes and metabolites. RESULTS: We identified 21 smoking-related metabolites in the baseline investigation (18 in men and six in women, with three overlaps) enriched in amino acid and lipid pathways, which were significantly different between current smokers and never smokers. Moreover, 19 out of the 21 metabolites were found to be reversible in former smokers. In the follow-up study, 13 reversible metabolites in men were measured, of which 10 were confirmed to be reversible in male quitters. Protein-metabolite networks are proposed to explain the consistent reversibility of smoking effects on metabolites. CONCLUSIONS: We showed that smoking-related changes in human serum metabolites are reversible after smoking cessation, consistent with the known cardiovascular risk reduction. The metabolites identified may serve as potential biomarkers to evaluate the status of smoking cessation and characterize smoking-related diseases. BioMed Central 2013-03-04 /pmc/articles/PMC3653729/ /pubmed/23497222 http://dx.doi.org/10.1186/1741-7015-11-60 Text en Copyright © 2013 Xu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xu, Tao Holzapfel, Christina Dong, Xiao Bader, Erik Yu, Zhonghao Prehn, Cornelia Perstorfer, Katrin Jaremek, Marta Roemisch-Margl, Werner Rathmann, Wolfgang Li, Yixue Wichmann, H -Erich Wallaschofski, Henri Ladwig, Karl H Theis, Fabian Suhre, Karsten Adamski, Jerzy Illig, Thomas Peters, Annette Wang-Sattler, Rui Effects of smoking and smoking cessation on human serum metabolite profile: results from the KORA cohort study |
title | Effects of smoking and smoking cessation on human serum metabolite profile: results from the KORA cohort study |
title_full | Effects of smoking and smoking cessation on human serum metabolite profile: results from the KORA cohort study |
title_fullStr | Effects of smoking and smoking cessation on human serum metabolite profile: results from the KORA cohort study |
title_full_unstemmed | Effects of smoking and smoking cessation on human serum metabolite profile: results from the KORA cohort study |
title_short | Effects of smoking and smoking cessation on human serum metabolite profile: results from the KORA cohort study |
title_sort | effects of smoking and smoking cessation on human serum metabolite profile: results from the kora cohort study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653729/ https://www.ncbi.nlm.nih.gov/pubmed/23497222 http://dx.doi.org/10.1186/1741-7015-11-60 |
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