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TREX exposes the RNA binding domain of Nxf1 to enable mRNA export
The metazoan TREX complex is recruited to mRNA during nuclear RNA processing and functions in exporting mRNA to the cytoplasm. Nxf1 is an mRNA export receptor, which binds processed mRNA and transports it through the nuclear pore complex. At present, the relationship between TREX and Nxf1 is not und...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654228/ https://www.ncbi.nlm.nih.gov/pubmed/22893130 http://dx.doi.org/10.1038/ncomms2005 |
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author | Viphakone, Nicolas Hautbergue, Guillaume M. Walsh, Matthew Chang, Chung-Te Holland, Arthur Folco, Eric G. Reed, Robin Wilson, Stuart A. |
author_facet | Viphakone, Nicolas Hautbergue, Guillaume M. Walsh, Matthew Chang, Chung-Te Holland, Arthur Folco, Eric G. Reed, Robin Wilson, Stuart A. |
author_sort | Viphakone, Nicolas |
collection | PubMed |
description | The metazoan TREX complex is recruited to mRNA during nuclear RNA processing and functions in exporting mRNA to the cytoplasm. Nxf1 is an mRNA export receptor, which binds processed mRNA and transports it through the nuclear pore complex. At present, the relationship between TREX and Nxf1 is not understood. Here we show that Nxf1 uses an intramolecular interaction to inhibit its own RNA binding activity. When the TREX subunits Aly and Thoc5 make contact with Nxf1, Nxf1 is driven into an open conformation, exposing its RNA binding domain, allowing RNA binding. Moreover, the combined knockdown of Aly and Thoc5 drastically reduces the amount of Nxf1 bound to mRNA in vivo and also causes a severe mRNA export block. Together, our data indicate that TREX provides a license for mRNA export by driving Nxf1 into a conformation capable of binding mRNA. |
format | Online Article Text |
id | pubmed-3654228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
record_format | MEDLINE/PubMed |
spelling | pubmed-36542282013-05-15 TREX exposes the RNA binding domain of Nxf1 to enable mRNA export Viphakone, Nicolas Hautbergue, Guillaume M. Walsh, Matthew Chang, Chung-Te Holland, Arthur Folco, Eric G. Reed, Robin Wilson, Stuart A. Nat Commun Article The metazoan TREX complex is recruited to mRNA during nuclear RNA processing and functions in exporting mRNA to the cytoplasm. Nxf1 is an mRNA export receptor, which binds processed mRNA and transports it through the nuclear pore complex. At present, the relationship between TREX and Nxf1 is not understood. Here we show that Nxf1 uses an intramolecular interaction to inhibit its own RNA binding activity. When the TREX subunits Aly and Thoc5 make contact with Nxf1, Nxf1 is driven into an open conformation, exposing its RNA binding domain, allowing RNA binding. Moreover, the combined knockdown of Aly and Thoc5 drastically reduces the amount of Nxf1 bound to mRNA in vivo and also causes a severe mRNA export block. Together, our data indicate that TREX provides a license for mRNA export by driving Nxf1 into a conformation capable of binding mRNA. 2012 /pmc/articles/PMC3654228/ /pubmed/22893130 http://dx.doi.org/10.1038/ncomms2005 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Viphakone, Nicolas Hautbergue, Guillaume M. Walsh, Matthew Chang, Chung-Te Holland, Arthur Folco, Eric G. Reed, Robin Wilson, Stuart A. TREX exposes the RNA binding domain of Nxf1 to enable mRNA export |
title | TREX exposes the RNA binding domain of Nxf1 to enable mRNA export |
title_full | TREX exposes the RNA binding domain of Nxf1 to enable mRNA export |
title_fullStr | TREX exposes the RNA binding domain of Nxf1 to enable mRNA export |
title_full_unstemmed | TREX exposes the RNA binding domain of Nxf1 to enable mRNA export |
title_short | TREX exposes the RNA binding domain of Nxf1 to enable mRNA export |
title_sort | trex exposes the rna binding domain of nxf1 to enable mrna export |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654228/ https://www.ncbi.nlm.nih.gov/pubmed/22893130 http://dx.doi.org/10.1038/ncomms2005 |
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