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Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference

In barley (Hordeum vulgare L.), chiasmata (the physical sites of genetic crossovers) are skewed towards the distal ends of chromosomes, effectively consigning a large proportion of genes to recombination coldspots. This has the effect of limiting potential genetic variability, and of reducing the ef...

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Autores principales: Phillips, Dylan, Wnetrzak, Joanna, Nibau, Candida, Barakate, Abdellah, Ramsay, Luke, Wright, Frank, Higgins, James D., Perry, Ruth M., Jenkins, Glyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654414/
https://www.ncbi.nlm.nih.gov/pubmed/23554258
http://dx.doi.org/10.1093/jxb/ert079
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author Phillips, Dylan
Wnetrzak, Joanna
Nibau, Candida
Barakate, Abdellah
Ramsay, Luke
Wright, Frank
Higgins, James D.
Perry, Ruth M.
Jenkins, Glyn
author_facet Phillips, Dylan
Wnetrzak, Joanna
Nibau, Candida
Barakate, Abdellah
Ramsay, Luke
Wright, Frank
Higgins, James D.
Perry, Ruth M.
Jenkins, Glyn
author_sort Phillips, Dylan
collection PubMed
description In barley (Hordeum vulgare L.), chiasmata (the physical sites of genetic crossovers) are skewed towards the distal ends of chromosomes, effectively consigning a large proportion of genes to recombination coldspots. This has the effect of limiting potential genetic variability, and of reducing the efficiency of map-based cloning and breeding approaches for this crop. Shifting the sites of recombination to more proximal chromosome regions by forward and reverse genetic means may be profitable in terms of realizing the genetic potential of the species, but is predicated upon a better understanding of the mechanisms governing the sites of these events, and upon the ability to recognize real changes in recombination patterns. The barley MutL Homologue (HvMLH3), a marker for class I interfering crossovers, has been isolated and a specific antibody has been raised. Immunolocalization of HvMLH3 along with the synaptonemal complex transverse filament protein ZYP1, used in conjunction with fluorescence in situ hybridization (FISH) tagging of specific barley chromosomes, has enabled access to the physical recombination landscape of the barley cultivars Morex and Bowman. Consistent distal localization of HvMLH3 foci throughout the genome, and similar patterns of HvMLH3 foci within bivalents 2H and 3H have been observed. A difference in total numbers of HvMLH3 foci between these two cultivars has been quantified, which is interpreted as representing genotypic variation in class I crossover frequency. Discrepancies between the frequencies of HvMLH3 foci and crossover frequencies derived from linkage analysis point to the existence of at least two crossover pathways in barley. It is also shown that interference of HvMLH3 foci is relatively weak compared with other plant species.
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spelling pubmed-36544142013-05-17 Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference Phillips, Dylan Wnetrzak, Joanna Nibau, Candida Barakate, Abdellah Ramsay, Luke Wright, Frank Higgins, James D. Perry, Ruth M. Jenkins, Glyn J Exp Bot Research Paper In barley (Hordeum vulgare L.), chiasmata (the physical sites of genetic crossovers) are skewed towards the distal ends of chromosomes, effectively consigning a large proportion of genes to recombination coldspots. This has the effect of limiting potential genetic variability, and of reducing the efficiency of map-based cloning and breeding approaches for this crop. Shifting the sites of recombination to more proximal chromosome regions by forward and reverse genetic means may be profitable in terms of realizing the genetic potential of the species, but is predicated upon a better understanding of the mechanisms governing the sites of these events, and upon the ability to recognize real changes in recombination patterns. The barley MutL Homologue (HvMLH3), a marker for class I interfering crossovers, has been isolated and a specific antibody has been raised. Immunolocalization of HvMLH3 along with the synaptonemal complex transverse filament protein ZYP1, used in conjunction with fluorescence in situ hybridization (FISH) tagging of specific barley chromosomes, has enabled access to the physical recombination landscape of the barley cultivars Morex and Bowman. Consistent distal localization of HvMLH3 foci throughout the genome, and similar patterns of HvMLH3 foci within bivalents 2H and 3H have been observed. A difference in total numbers of HvMLH3 foci between these two cultivars has been quantified, which is interpreted as representing genotypic variation in class I crossover frequency. Discrepancies between the frequencies of HvMLH3 foci and crossover frequencies derived from linkage analysis point to the existence of at least two crossover pathways in barley. It is also shown that interference of HvMLH3 foci is relatively weak compared with other plant species. Oxford University Press 2013-05 2013-04-03 /pmc/articles/PMC3654414/ /pubmed/23554258 http://dx.doi.org/10.1093/jxb/ert079 Text en © The Author(2) [2013]. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Phillips, Dylan
Wnetrzak, Joanna
Nibau, Candida
Barakate, Abdellah
Ramsay, Luke
Wright, Frank
Higgins, James D.
Perry, Ruth M.
Jenkins, Glyn
Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference
title Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference
title_full Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference
title_fullStr Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference
title_full_unstemmed Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference
title_short Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference
title_sort quantitative high resolution mapping of hvmlh3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654414/
https://www.ncbi.nlm.nih.gov/pubmed/23554258
http://dx.doi.org/10.1093/jxb/ert079
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