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An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration

Dehydration is a major factor resulting in huge loss from cut flowers during transportation. In the present study, dehydration inhibited petal cell expansion and resulted in irregular flowers in cut roses, mimicking ethylene-treated flowers. Among the five floral organs, dehydration substantially el...

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Autores principales: Liu, Daofeng, Liu, Xiaojing, Meng, Yonglu, Sun, Cuihui, Tang, Hongshu, Jiang, Yudong, Khan, Muhammad Ali, Xue, Jingqi, Ma, Nan, Gao, Junping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654423/
https://www.ncbi.nlm.nih.gov/pubmed/23599274
http://dx.doi.org/10.1093/jxb/ert092
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author Liu, Daofeng
Liu, Xiaojing
Meng, Yonglu
Sun, Cuihui
Tang, Hongshu
Jiang, Yudong
Khan, Muhammad Ali
Xue, Jingqi
Ma, Nan
Gao, Junping
author_facet Liu, Daofeng
Liu, Xiaojing
Meng, Yonglu
Sun, Cuihui
Tang, Hongshu
Jiang, Yudong
Khan, Muhammad Ali
Xue, Jingqi
Ma, Nan
Gao, Junping
author_sort Liu, Daofeng
collection PubMed
description Dehydration is a major factor resulting in huge loss from cut flowers during transportation. In the present study, dehydration inhibited petal cell expansion and resulted in irregular flowers in cut roses, mimicking ethylene-treated flowers. Among the five floral organs, dehydration substantially elevated ethylene production in the sepals, whilst rehydration caused rapid and elevated ethylene levels in the gynoecia and sepals. Among the five ethylene biosynthetic enzyme genes (RhACS1–5), expression of RhACS1 and RhACS2 was induced by dehydration and rehydration in the two floral organs. Silencing both RhACS1 and RhACS2 significantly suppressed dehydration- and rehydration-induced ethylene in the sepals and gynoecia. This weakened the inhibitory effect of dehydration on petal cell expansion. β-glucuronidase activity driven by both the RhACS1 and RhACS2 promoters was dramatically induced in the sepals, pistil, and stamens, but not in the petals of transgenic Arabidopsis. This further supports the organ-specific induction of these two genes. Among the five rose ethylene receptor genes (RhETR1–5), expression of RhETR3 was predominantly induced by dehydration and rehydration in the petals. RhETR3 silencing clearly aggravated the inhibitory effect of dehydration on petal cell expansion. However, no significant difference in the effect between RhETR3-silenced flowers and RhETR-genes-silenced flowers was observed. Furthermore, RhETR-genes silencing extensively altered the expression of 21 cell expansion-related downstream genes in response to ethylene. These results suggest that induction of ethylene biosynthesis by dehydration proceeds in an organ-specific manner, indicating that ethylene can function as a mediator in dehydration-caused inhibition of cell expansion in rose petals.
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spelling pubmed-36544232014-05-01 An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration Liu, Daofeng Liu, Xiaojing Meng, Yonglu Sun, Cuihui Tang, Hongshu Jiang, Yudong Khan, Muhammad Ali Xue, Jingqi Ma, Nan Gao, Junping J Exp Bot Research Paper Dehydration is a major factor resulting in huge loss from cut flowers during transportation. In the present study, dehydration inhibited petal cell expansion and resulted in irregular flowers in cut roses, mimicking ethylene-treated flowers. Among the five floral organs, dehydration substantially elevated ethylene production in the sepals, whilst rehydration caused rapid and elevated ethylene levels in the gynoecia and sepals. Among the five ethylene biosynthetic enzyme genes (RhACS1–5), expression of RhACS1 and RhACS2 was induced by dehydration and rehydration in the two floral organs. Silencing both RhACS1 and RhACS2 significantly suppressed dehydration- and rehydration-induced ethylene in the sepals and gynoecia. This weakened the inhibitory effect of dehydration on petal cell expansion. β-glucuronidase activity driven by both the RhACS1 and RhACS2 promoters was dramatically induced in the sepals, pistil, and stamens, but not in the petals of transgenic Arabidopsis. This further supports the organ-specific induction of these two genes. Among the five rose ethylene receptor genes (RhETR1–5), expression of RhETR3 was predominantly induced by dehydration and rehydration in the petals. RhETR3 silencing clearly aggravated the inhibitory effect of dehydration on petal cell expansion. However, no significant difference in the effect between RhETR3-silenced flowers and RhETR-genes-silenced flowers was observed. Furthermore, RhETR-genes silencing extensively altered the expression of 21 cell expansion-related downstream genes in response to ethylene. These results suggest that induction of ethylene biosynthesis by dehydration proceeds in an organ-specific manner, indicating that ethylene can function as a mediator in dehydration-caused inhibition of cell expansion in rose petals. Oxford University Press 2013-05 2013-04-18 /pmc/articles/PMC3654423/ /pubmed/23599274 http://dx.doi.org/10.1093/jxb/ert092 Text en © The Author(2) [2013]. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.
spellingShingle Research Paper
Liu, Daofeng
Liu, Xiaojing
Meng, Yonglu
Sun, Cuihui
Tang, Hongshu
Jiang, Yudong
Khan, Muhammad Ali
Xue, Jingqi
Ma, Nan
Gao, Junping
An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration
title An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration
title_full An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration
title_fullStr An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration
title_full_unstemmed An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration
title_short An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration
title_sort organ-specific role for ethylene in rose petal expansion during dehydration and rehydration
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654423/
https://www.ncbi.nlm.nih.gov/pubmed/23599274
http://dx.doi.org/10.1093/jxb/ert092
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