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ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast
Mitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER–mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of these contacts are not understood. Here we show that in yeast, the ER–mitochondria...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654481/ https://www.ncbi.nlm.nih.gov/pubmed/23682313 http://dx.doi.org/10.7554/eLife.00422 |
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author | Murley, Andrew Lackner, Laura L Osman, Christof West, Matthew Voeltz, Gia K Walter, Peter Nunnari, Jodi |
author_facet | Murley, Andrew Lackner, Laura L Osman, Christof West, Matthew Voeltz, Gia K Walter, Peter Nunnari, Jodi |
author_sort | Murley, Andrew |
collection | PubMed |
description | Mitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER–mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of these contacts are not understood. Here we show that in yeast, the ER–mitochondria tethering complex, ERMES, and the highly conserved Miro GTPase, Gem1, are spatially and functionally linked to ER-associated mitochondrial division. Gem1 acts as a negative regulator of ER–mitochondria contacts, an activity required for the spatial resolution and distribution of newly generated mitochondrial tips following division. Previous data have demonstrated that ERMES localizes with a subset of actively replicating mitochondrial nucleoids. We show that mitochondrial division is spatially linked to nucleoids and that a majority of these nucleoids segregate prior to division, resulting in their distribution into newly generated tips in the mitochondrial network. Thus, we postulate that ER-associated division serves to link the distribution of mitochondria and mitochondrial nucleoids in cells. DOI: http://dx.doi.org/10.7554/eLife.00422.001 |
format | Online Article Text |
id | pubmed-3654481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-36544812013-05-16 ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast Murley, Andrew Lackner, Laura L Osman, Christof West, Matthew Voeltz, Gia K Walter, Peter Nunnari, Jodi eLife Cell Biology Mitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER–mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of these contacts are not understood. Here we show that in yeast, the ER–mitochondria tethering complex, ERMES, and the highly conserved Miro GTPase, Gem1, are spatially and functionally linked to ER-associated mitochondrial division. Gem1 acts as a negative regulator of ER–mitochondria contacts, an activity required for the spatial resolution and distribution of newly generated mitochondrial tips following division. Previous data have demonstrated that ERMES localizes with a subset of actively replicating mitochondrial nucleoids. We show that mitochondrial division is spatially linked to nucleoids and that a majority of these nucleoids segregate prior to division, resulting in their distribution into newly generated tips in the mitochondrial network. Thus, we postulate that ER-associated division serves to link the distribution of mitochondria and mitochondrial nucleoids in cells. DOI: http://dx.doi.org/10.7554/eLife.00422.001 eLife Sciences Publications, Ltd 2013-05-14 /pmc/articles/PMC3654481/ /pubmed/23682313 http://dx.doi.org/10.7554/eLife.00422 Text en Copyright © 2013, Murley et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Murley, Andrew Lackner, Laura L Osman, Christof West, Matthew Voeltz, Gia K Walter, Peter Nunnari, Jodi ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
title | ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
title_full | ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
title_fullStr | ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
title_full_unstemmed | ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
title_short | ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast |
title_sort | er-associated mitochondrial division links the distribution of mitochondria and mitochondrial dna in yeast |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654481/ https://www.ncbi.nlm.nih.gov/pubmed/23682313 http://dx.doi.org/10.7554/eLife.00422 |
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