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ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast

Mitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER–mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of these contacts are not understood. Here we show that in yeast, the ER–mitochondria...

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Autores principales: Murley, Andrew, Lackner, Laura L, Osman, Christof, West, Matthew, Voeltz, Gia K, Walter, Peter, Nunnari, Jodi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654481/
https://www.ncbi.nlm.nih.gov/pubmed/23682313
http://dx.doi.org/10.7554/eLife.00422
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author Murley, Andrew
Lackner, Laura L
Osman, Christof
West, Matthew
Voeltz, Gia K
Walter, Peter
Nunnari, Jodi
author_facet Murley, Andrew
Lackner, Laura L
Osman, Christof
West, Matthew
Voeltz, Gia K
Walter, Peter
Nunnari, Jodi
author_sort Murley, Andrew
collection PubMed
description Mitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER–mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of these contacts are not understood. Here we show that in yeast, the ER–mitochondria tethering complex, ERMES, and the highly conserved Miro GTPase, Gem1, are spatially and functionally linked to ER-associated mitochondrial division. Gem1 acts as a negative regulator of ER–mitochondria contacts, an activity required for the spatial resolution and distribution of newly generated mitochondrial tips following division. Previous data have demonstrated that ERMES localizes with a subset of actively replicating mitochondrial nucleoids. We show that mitochondrial division is spatially linked to nucleoids and that a majority of these nucleoids segregate prior to division, resulting in their distribution into newly generated tips in the mitochondrial network. Thus, we postulate that ER-associated division serves to link the distribution of mitochondria and mitochondrial nucleoids in cells. DOI: http://dx.doi.org/10.7554/eLife.00422.001
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spelling pubmed-36544812013-05-16 ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast Murley, Andrew Lackner, Laura L Osman, Christof West, Matthew Voeltz, Gia K Walter, Peter Nunnari, Jodi eLife Cell Biology Mitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER–mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of these contacts are not understood. Here we show that in yeast, the ER–mitochondria tethering complex, ERMES, and the highly conserved Miro GTPase, Gem1, are spatially and functionally linked to ER-associated mitochondrial division. Gem1 acts as a negative regulator of ER–mitochondria contacts, an activity required for the spatial resolution and distribution of newly generated mitochondrial tips following division. Previous data have demonstrated that ERMES localizes with a subset of actively replicating mitochondrial nucleoids. We show that mitochondrial division is spatially linked to nucleoids and that a majority of these nucleoids segregate prior to division, resulting in their distribution into newly generated tips in the mitochondrial network. Thus, we postulate that ER-associated division serves to link the distribution of mitochondria and mitochondrial nucleoids in cells. DOI: http://dx.doi.org/10.7554/eLife.00422.001 eLife Sciences Publications, Ltd 2013-05-14 /pmc/articles/PMC3654481/ /pubmed/23682313 http://dx.doi.org/10.7554/eLife.00422 Text en Copyright © 2013, Murley et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Murley, Andrew
Lackner, Laura L
Osman, Christof
West, Matthew
Voeltz, Gia K
Walter, Peter
Nunnari, Jodi
ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast
title ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast
title_full ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast
title_fullStr ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast
title_full_unstemmed ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast
title_short ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast
title_sort er-associated mitochondrial division links the distribution of mitochondria and mitochondrial dna in yeast
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654481/
https://www.ncbi.nlm.nih.gov/pubmed/23682313
http://dx.doi.org/10.7554/eLife.00422
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