Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, w...

Descripción completa

Detalles Bibliográficos
Autores principales: Querido, Jailson F. B., Agirre, Jon, Marti, Gerardo A., Guérin, Diego M. A., Silva, Marcelo Sousa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Methodology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654624/
https://www.ncbi.nlm.nih.gov/pubmed/23710438
http://dx.doi.org/10.1155/2013/218593
Descripción
Sumario:Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.

Ejemplares similares