Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, w...

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Detalles Bibliográficos
Autores principales: Querido, Jailson F. B., Agirre, Jon, Marti, Gerardo A., Guérin, Diego M. A., Silva, Marcelo Sousa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Methodology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654624/
https://www.ncbi.nlm.nih.gov/pubmed/23710438
http://dx.doi.org/10.1155/2013/218593
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author Querido, Jailson F. B.
Agirre, Jon
Marti, Gerardo A.
Guérin, Diego M. A.
Silva, Marcelo Sousa
author_facet Querido, Jailson F. B.
Agirre, Jon
Marti, Gerardo A.
Guérin, Diego M. A.
Silva, Marcelo Sousa
author_sort Querido, Jailson F. B.
collection PubMed
description Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
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spelling pubmed-36546242013-05-24 Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification Querido, Jailson F. B. Agirre, Jon Marti, Gerardo A. Guérin, Diego M. A. Silva, Marcelo Sousa Biomed Res Int Methodology Report Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus. Hindawi Publishing Corporation 2013 2013-04-04 /pmc/articles/PMC3654624/ /pubmed/23710438 http://dx.doi.org/10.1155/2013/218593 Text en Copyright © 2013 Jailson F. B. Querido et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Report
Querido, Jailson F. B.
Agirre, Jon
Marti, Gerardo A.
Guérin, Diego M. A.
Silva, Marcelo Sousa
Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_full Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_fullStr Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_full_unstemmed Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_short Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
title_sort molecular techniques for dicistrovirus detection without rna extraction or purification
topic Methodology Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654624/
https://www.ncbi.nlm.nih.gov/pubmed/23710438
http://dx.doi.org/10.1155/2013/218593
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