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A comparison across non-model animals suggests an optimal sequencing depth for de novo transcriptome assembly

BACKGROUND: The lack of genomic resources can present challenges for studies of non-model organisms. Transcriptome sequencing offers an attractive method to gather information about genes and gene expression without the need for a reference genome. However, it is unclear what sequencing depth is ade...

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Detalles Bibliográficos
Autores principales: Francis, Warren R, Christianson, Lynne M, Kiko, Rainer, Powers, Meghan L, Shaner, Nathan C, D Haddock, Steven H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655071/
https://www.ncbi.nlm.nih.gov/pubmed/23496952
http://dx.doi.org/10.1186/1471-2164-14-167