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Tagging the Expressed Protein with 6 Histidines: Rapid Cloning of an Amplicon with Three Options
We report the designing of three expression vectors that can be used for rapid cloning of any blunt-end DNA segment. Only a single set of oligonucleotides are required to perform the amplification of the target DNA and its cloning in all three vectors simultaneously. The DNA thus cloned can express...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655076/ https://www.ncbi.nlm.nih.gov/pubmed/23691118 http://dx.doi.org/10.1371/journal.pone.0063922 |