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Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil
Leptodactylus macrosternum and L. vastus (family: Leptodactylidae) are commonly encountered in the “Caatinga” biome in northern Brazil. The body fat of L. vastus is used as a zootherapeutic for treating a number of human maladies. The aim of this work was to determine the chemical composition of the...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655571/ https://www.ncbi.nlm.nih.gov/pubmed/23710241 http://dx.doi.org/10.1155/2013/913671 |
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author | Cabral, Mario Eduardo Santos Dias, Diógenes de Queiroz Sales, Débora Lima Oliveira, Olga Paiva Teles, Diego Alves Filho, João Antonio de Araujo de Sousa, José Guilherme Gonçalves Coutinho, Henrique Douglas Melo da Costa, José Galberto Martins Kerntopf, Marta Regina Alves, Rômulo Romeu da Nóbrega Almeida, Waltécio de Oliveira |
author_facet | Cabral, Mario Eduardo Santos Dias, Diógenes de Queiroz Sales, Débora Lima Oliveira, Olga Paiva Teles, Diego Alves Filho, João Antonio de Araujo de Sousa, José Guilherme Gonçalves Coutinho, Henrique Douglas Melo da Costa, José Galberto Martins Kerntopf, Marta Regina Alves, Rômulo Romeu da Nóbrega Almeida, Waltécio de Oliveira |
author_sort | Cabral, Mario Eduardo Santos |
collection | PubMed |
description | Leptodactylus macrosternum and L. vastus (family: Leptodactylidae) are commonly encountered in the “Caatinga” biome in northern Brazil. The body fat of L. vastus is used as a zootherapeutic for treating a number of human maladies. The aim of this work was to determine the chemical composition of the body fats of L. macrosternum and L. vastus and to evaluate their antimicrobial activities as well as the ecological implications of their use in traditional folk medicine. Oils were extracted from body fat located in the ventral region of L. macrosternum (OLM) and L. vastus (OLV) using hexane as a solvent. The fatty acids were identified by GC-MS. The antimicrobial activities of the oils, either alone or in combination with antibiotics and antifungal drugs, were tested on standard strains of microorganisms as well as on multiresistant strains of Escherichia coli and Staphylococcus. OLM contained 40% saturated and 60% unsaturated fatty acids, while OLV contained 58.33% saturated and 41.67% unsaturated fatty acids. Our results indicated that both OLM and OLV demonstrated relevant antimicrobial activities (with MIC 256 μg/mL for both) against Pseudomonas aeruginosa and Candida krusei. However, no antimicrobial effects were observed when these oils were combined with antibiotics or antifungal drugs. |
format | Online Article Text |
id | pubmed-3655571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36555712013-05-24 Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil Cabral, Mario Eduardo Santos Dias, Diógenes de Queiroz Sales, Débora Lima Oliveira, Olga Paiva Teles, Diego Alves Filho, João Antonio de Araujo de Sousa, José Guilherme Gonçalves Coutinho, Henrique Douglas Melo da Costa, José Galberto Martins Kerntopf, Marta Regina Alves, Rômulo Romeu da Nóbrega Almeida, Waltécio de Oliveira Evid Based Complement Alternat Med Research Article Leptodactylus macrosternum and L. vastus (family: Leptodactylidae) are commonly encountered in the “Caatinga” biome in northern Brazil. The body fat of L. vastus is used as a zootherapeutic for treating a number of human maladies. The aim of this work was to determine the chemical composition of the body fats of L. macrosternum and L. vastus and to evaluate their antimicrobial activities as well as the ecological implications of their use in traditional folk medicine. Oils were extracted from body fat located in the ventral region of L. macrosternum (OLM) and L. vastus (OLV) using hexane as a solvent. The fatty acids were identified by GC-MS. The antimicrobial activities of the oils, either alone or in combination with antibiotics and antifungal drugs, were tested on standard strains of microorganisms as well as on multiresistant strains of Escherichia coli and Staphylococcus. OLM contained 40% saturated and 60% unsaturated fatty acids, while OLV contained 58.33% saturated and 41.67% unsaturated fatty acids. Our results indicated that both OLM and OLV demonstrated relevant antimicrobial activities (with MIC 256 μg/mL for both) against Pseudomonas aeruginosa and Candida krusei. However, no antimicrobial effects were observed when these oils were combined with antibiotics or antifungal drugs. Hindawi Publishing Corporation 2013 2013-04-23 /pmc/articles/PMC3655571/ /pubmed/23710241 http://dx.doi.org/10.1155/2013/913671 Text en Copyright © 2013 Mario Eduardo Santos Cabral et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cabral, Mario Eduardo Santos Dias, Diógenes de Queiroz Sales, Débora Lima Oliveira, Olga Paiva Teles, Diego Alves Filho, João Antonio de Araujo de Sousa, José Guilherme Gonçalves Coutinho, Henrique Douglas Melo da Costa, José Galberto Martins Kerntopf, Marta Regina Alves, Rômulo Romeu da Nóbrega Almeida, Waltécio de Oliveira Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil |
title | Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil |
title_full | Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil |
title_fullStr | Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil |
title_full_unstemmed | Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil |
title_short | Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil |
title_sort | evaluations of the antimicrobial activities and chemical compositions of body fat from the amphibians leptodactylus macrosternum miranda-ribeiro (1926) and leptodactylus vastus adolf lutz (1930) in northeastern brazil |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655571/ https://www.ncbi.nlm.nih.gov/pubmed/23710241 http://dx.doi.org/10.1155/2013/913671 |
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