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Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil

Leptodactylus macrosternum and L. vastus (family: Leptodactylidae) are commonly encountered in the “Caatinga” biome in northern Brazil. The body fat of L. vastus is used as a zootherapeutic for treating a number of human maladies. The aim of this work was to determine the chemical composition of the...

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Autores principales: Cabral, Mario Eduardo Santos, Dias, Diógenes de Queiroz, Sales, Débora Lima, Oliveira, Olga Paiva, Teles, Diego Alves, Filho, João Antonio de Araujo, de Sousa, José Guilherme Gonçalves, Coutinho, Henrique Douglas Melo, da Costa, José Galberto Martins, Kerntopf, Marta Regina, Alves, Rômulo Romeu da Nóbrega, Almeida, Waltécio de Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655571/
https://www.ncbi.nlm.nih.gov/pubmed/23710241
http://dx.doi.org/10.1155/2013/913671
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author Cabral, Mario Eduardo Santos
Dias, Diógenes de Queiroz
Sales, Débora Lima
Oliveira, Olga Paiva
Teles, Diego Alves
Filho, João Antonio de Araujo
de Sousa, José Guilherme Gonçalves
Coutinho, Henrique Douglas Melo
da Costa, José Galberto Martins
Kerntopf, Marta Regina
Alves, Rômulo Romeu da Nóbrega
Almeida, Waltécio de Oliveira
author_facet Cabral, Mario Eduardo Santos
Dias, Diógenes de Queiroz
Sales, Débora Lima
Oliveira, Olga Paiva
Teles, Diego Alves
Filho, João Antonio de Araujo
de Sousa, José Guilherme Gonçalves
Coutinho, Henrique Douglas Melo
da Costa, José Galberto Martins
Kerntopf, Marta Regina
Alves, Rômulo Romeu da Nóbrega
Almeida, Waltécio de Oliveira
author_sort Cabral, Mario Eduardo Santos
collection PubMed
description Leptodactylus macrosternum and L. vastus (family: Leptodactylidae) are commonly encountered in the “Caatinga” biome in northern Brazil. The body fat of L. vastus is used as a zootherapeutic for treating a number of human maladies. The aim of this work was to determine the chemical composition of the body fats of L. macrosternum and L. vastus and to evaluate their antimicrobial activities as well as the ecological implications of their use in traditional folk medicine. Oils were extracted from body fat located in the ventral region of L. macrosternum (OLM) and L. vastus (OLV) using hexane as a solvent. The fatty acids were identified by GC-MS. The antimicrobial activities of the oils, either alone or in combination with antibiotics and antifungal drugs, were tested on standard strains of microorganisms as well as on multiresistant strains of Escherichia coli and Staphylococcus. OLM contained 40% saturated and 60% unsaturated fatty acids, while OLV contained 58.33% saturated and 41.67% unsaturated fatty acids. Our results indicated that both OLM and OLV demonstrated relevant antimicrobial activities (with MIC 256 μg/mL for both) against Pseudomonas aeruginosa and Candida krusei. However, no antimicrobial effects were observed when these oils were combined with antibiotics or antifungal drugs.
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spelling pubmed-36555712013-05-24 Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil Cabral, Mario Eduardo Santos Dias, Diógenes de Queiroz Sales, Débora Lima Oliveira, Olga Paiva Teles, Diego Alves Filho, João Antonio de Araujo de Sousa, José Guilherme Gonçalves Coutinho, Henrique Douglas Melo da Costa, José Galberto Martins Kerntopf, Marta Regina Alves, Rômulo Romeu da Nóbrega Almeida, Waltécio de Oliveira Evid Based Complement Alternat Med Research Article Leptodactylus macrosternum and L. vastus (family: Leptodactylidae) are commonly encountered in the “Caatinga” biome in northern Brazil. The body fat of L. vastus is used as a zootherapeutic for treating a number of human maladies. The aim of this work was to determine the chemical composition of the body fats of L. macrosternum and L. vastus and to evaluate their antimicrobial activities as well as the ecological implications of their use in traditional folk medicine. Oils were extracted from body fat located in the ventral region of L. macrosternum (OLM) and L. vastus (OLV) using hexane as a solvent. The fatty acids were identified by GC-MS. The antimicrobial activities of the oils, either alone or in combination with antibiotics and antifungal drugs, were tested on standard strains of microorganisms as well as on multiresistant strains of Escherichia coli and Staphylococcus. OLM contained 40% saturated and 60% unsaturated fatty acids, while OLV contained 58.33% saturated and 41.67% unsaturated fatty acids. Our results indicated that both OLM and OLV demonstrated relevant antimicrobial activities (with MIC 256 μg/mL for both) against Pseudomonas aeruginosa and Candida krusei. However, no antimicrobial effects were observed when these oils were combined with antibiotics or antifungal drugs. Hindawi Publishing Corporation 2013 2013-04-23 /pmc/articles/PMC3655571/ /pubmed/23710241 http://dx.doi.org/10.1155/2013/913671 Text en Copyright © 2013 Mario Eduardo Santos Cabral et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cabral, Mario Eduardo Santos
Dias, Diógenes de Queiroz
Sales, Débora Lima
Oliveira, Olga Paiva
Teles, Diego Alves
Filho, João Antonio de Araujo
de Sousa, José Guilherme Gonçalves
Coutinho, Henrique Douglas Melo
da Costa, José Galberto Martins
Kerntopf, Marta Regina
Alves, Rômulo Romeu da Nóbrega
Almeida, Waltécio de Oliveira
Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil
title Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil
title_full Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil
title_fullStr Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil
title_full_unstemmed Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil
title_short Evaluations of the Antimicrobial Activities and Chemical Compositions of Body Fat from the Amphibians Leptodactylus macrosternum Miranda-Ribeiro (1926) and Leptodactylus vastus Adolf Lutz (1930) in Northeastern Brazil
title_sort evaluations of the antimicrobial activities and chemical compositions of body fat from the amphibians leptodactylus macrosternum miranda-ribeiro (1926) and leptodactylus vastus adolf lutz (1930) in northeastern brazil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655571/
https://www.ncbi.nlm.nih.gov/pubmed/23710241
http://dx.doi.org/10.1155/2013/913671
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