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Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula
Aim. The objective of this study was to characterize coordinated molecular changes in the structure and composition of the walls of venous segments of arteriovenous (AV) fistulas evoked by overflow. Methods. Venous tissue samples were collected from 6 hemodialysis patients with AV fistulas exposed t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655589/ https://www.ncbi.nlm.nih.gov/pubmed/23710358 http://dx.doi.org/10.1155/2013/980923 |
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author | Hashimoto, Yasuhiro Okamoto, Akiko Saitoh, Hisao Hatakeyama, Shingo Yoneyama, Takahiro Koie, Takuya Ohyama, Chikara |
author_facet | Hashimoto, Yasuhiro Okamoto, Akiko Saitoh, Hisao Hatakeyama, Shingo Yoneyama, Takahiro Koie, Takuya Ohyama, Chikara |
author_sort | Hashimoto, Yasuhiro |
collection | PubMed |
description | Aim. The objective of this study was to characterize coordinated molecular changes in the structure and composition of the walls of venous segments of arteriovenous (AV) fistulas evoked by overflow. Methods. Venous tissue samples were collected from 6 hemodialysis patients with AV fistulas exposed to overflow and from the normal cephalic veins of 4 other hemodialysis patients. Total RNA was extracted from the venous tissue samples, and gene expression between the 2 groups was compared using Whole Human Genome DNA microarray 44 K. Microarray data were analyzed by GeneSpring GX software and Ingenuity Pathway Analysis. Results. The cDNA microarray analysis identified 397 upregulated genes and 456 downregulated genes. Gene ontology analysis with GeneSpring GX software revealed that biological developmental processes and glycosaminoglycan binding were the most upregulated. In addition, most upregulation occurred extracellularly. In the pathway analysis, the TGF beta signaling pathway, cytokines and inflammatory response pathway, hypertrophy model, and the myometrial relaxation and contraction pathway were significantly upregulated compared with the control cephalic vein. Conclusion. Combining microarray results and pathway information available via the Internet provided biological insight into the structure and composition of the venous wall of overflow AV fistulas. |
format | Online Article Text |
id | pubmed-3655589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36555892013-05-24 Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula Hashimoto, Yasuhiro Okamoto, Akiko Saitoh, Hisao Hatakeyama, Shingo Yoneyama, Takahiro Koie, Takuya Ohyama, Chikara Int J Nephrol Research Article Aim. The objective of this study was to characterize coordinated molecular changes in the structure and composition of the walls of venous segments of arteriovenous (AV) fistulas evoked by overflow. Methods. Venous tissue samples were collected from 6 hemodialysis patients with AV fistulas exposed to overflow and from the normal cephalic veins of 4 other hemodialysis patients. Total RNA was extracted from the venous tissue samples, and gene expression between the 2 groups was compared using Whole Human Genome DNA microarray 44 K. Microarray data were analyzed by GeneSpring GX software and Ingenuity Pathway Analysis. Results. The cDNA microarray analysis identified 397 upregulated genes and 456 downregulated genes. Gene ontology analysis with GeneSpring GX software revealed that biological developmental processes and glycosaminoglycan binding were the most upregulated. In addition, most upregulation occurred extracellularly. In the pathway analysis, the TGF beta signaling pathway, cytokines and inflammatory response pathway, hypertrophy model, and the myometrial relaxation and contraction pathway were significantly upregulated compared with the control cephalic vein. Conclusion. Combining microarray results and pathway information available via the Internet provided biological insight into the structure and composition of the venous wall of overflow AV fistulas. Hindawi Publishing Corporation 2013 2013-04-27 /pmc/articles/PMC3655589/ /pubmed/23710358 http://dx.doi.org/10.1155/2013/980923 Text en Copyright © 2013 Yasuhiro Hashimoto et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hashimoto, Yasuhiro Okamoto, Akiko Saitoh, Hisao Hatakeyama, Shingo Yoneyama, Takahiro Koie, Takuya Ohyama, Chikara Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula |
title | Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula |
title_full | Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula |
title_fullStr | Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula |
title_full_unstemmed | Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula |
title_short | Gene Expression Changes in Venous Segment of Overflow Arteriovenous Fistula |
title_sort | gene expression changes in venous segment of overflow arteriovenous fistula |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655589/ https://www.ncbi.nlm.nih.gov/pubmed/23710358 http://dx.doi.org/10.1155/2013/980923 |
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