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Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats

Many studies suggest that fluoride exposure can inhibit the activity of various enzymes and can generate free radicals, which interfere with antioxidant defence mechanisms in living systems. To further the understanding of this issue, this present study examined the effects of low-dose fluoride trea...

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Autores principales: Yamaguti, Paula Mochidome, Simões, Alyne, Ganzerla, Emily, Souza, Douglas Nesadal, Nogueira, Fernando Neves, Nicolau, José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655635/
https://www.ncbi.nlm.nih.gov/pubmed/23738039
http://dx.doi.org/10.1155/2013/674593
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author Yamaguti, Paula Mochidome
Simões, Alyne
Ganzerla, Emily
Souza, Douglas Nesadal
Nogueira, Fernando Neves
Nicolau, José
author_facet Yamaguti, Paula Mochidome
Simões, Alyne
Ganzerla, Emily
Souza, Douglas Nesadal
Nogueira, Fernando Neves
Nicolau, José
author_sort Yamaguti, Paula Mochidome
collection PubMed
description Many studies suggest that fluoride exposure can inhibit the activity of various enzymes and can generate free radicals, which interfere with antioxidant defence mechanisms in living systems. To further the understanding of this issue, this present study examined the effects of low-dose fluoride treatment on the activity of enzymatic antioxidant superoxide dismutase (SOD) and catalase (CAT), as well as the levels of lipid peroxidation (LPO) in the parotid (PA) and submandibular (SM) salivary glands of rats. Rats were injected with a single dose of sodium fluoride (NaF) (15 mg F(−)/kg b.w.) then euthanized at various time intervals up to 24 hours (h) following exposure. NaF exposure did not cause significant differences in SOD or CAT activity or LPO levels in PA glands compared to control. Conversely, SM glands presented increased SOD activity after 3 h and decreased SOD activity after 1, 12, and 24 h, while LPO was increased after 6, 12, and 24 h of the NaF injection. There were no significant differences in the CAT activity in the groups studied. Our results demonstrated that NaF intoxication caused oxidative stress in salivary glands few hours after administration. These changes were more pronounced in SM than in PA gland.
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spelling pubmed-36556352013-06-04 Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats Yamaguti, Paula Mochidome Simões, Alyne Ganzerla, Emily Souza, Douglas Nesadal Nogueira, Fernando Neves Nicolau, José Oxid Med Cell Longev Research Article Many studies suggest that fluoride exposure can inhibit the activity of various enzymes and can generate free radicals, which interfere with antioxidant defence mechanisms in living systems. To further the understanding of this issue, this present study examined the effects of low-dose fluoride treatment on the activity of enzymatic antioxidant superoxide dismutase (SOD) and catalase (CAT), as well as the levels of lipid peroxidation (LPO) in the parotid (PA) and submandibular (SM) salivary glands of rats. Rats were injected with a single dose of sodium fluoride (NaF) (15 mg F(−)/kg b.w.) then euthanized at various time intervals up to 24 hours (h) following exposure. NaF exposure did not cause significant differences in SOD or CAT activity or LPO levels in PA glands compared to control. Conversely, SM glands presented increased SOD activity after 3 h and decreased SOD activity after 1, 12, and 24 h, while LPO was increased after 6, 12, and 24 h of the NaF injection. There were no significant differences in the CAT activity in the groups studied. Our results demonstrated that NaF intoxication caused oxidative stress in salivary glands few hours after administration. These changes were more pronounced in SM than in PA gland. Hindawi Publishing Corporation 2013 2013-04-27 /pmc/articles/PMC3655635/ /pubmed/23738039 http://dx.doi.org/10.1155/2013/674593 Text en Copyright © 2013 Paula Mochidome Yamaguti et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yamaguti, Paula Mochidome
Simões, Alyne
Ganzerla, Emily
Souza, Douglas Nesadal
Nogueira, Fernando Neves
Nicolau, José
Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats
title Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats
title_full Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats
title_fullStr Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats
title_full_unstemmed Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats
title_short Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats
title_sort effects of single exposure of sodium fluoride on lipid peroxidation and antioxidant enzymes in salivary glands of rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655635/
https://www.ncbi.nlm.nih.gov/pubmed/23738039
http://dx.doi.org/10.1155/2013/674593
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