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NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata

BACKGROUND: To survive herbivore attack, plants have evolved potent mechanisms of mechanical or chemical defense that are either constitutively present or inducible after herbivore attack. Due to the costs of defense deployment, plants often regulate their biosynthesis using various transcription fa...

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Autores principales: Woldemariam, Melkamu G, Dinh, Son Truong, Oh, Youngjoo, Gaquerel, Emmanuel, Baldwin, Ian T, Galis, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655906/
https://www.ncbi.nlm.nih.gov/pubmed/23634896
http://dx.doi.org/10.1186/1471-2229-13-73
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author Woldemariam, Melkamu G
Dinh, Son Truong
Oh, Youngjoo
Gaquerel, Emmanuel
Baldwin, Ian T
Galis, Ivan
author_facet Woldemariam, Melkamu G
Dinh, Son Truong
Oh, Youngjoo
Gaquerel, Emmanuel
Baldwin, Ian T
Galis, Ivan
author_sort Woldemariam, Melkamu G
collection PubMed
description BACKGROUND: To survive herbivore attack, plants have evolved potent mechanisms of mechanical or chemical defense that are either constitutively present or inducible after herbivore attack. Due to the costs of defense deployment, plants often regulate their biosynthesis using various transcription factors (TFs). MYC2 regulators belong to the bHLH family of transcription factors that are involved in many aspects of plant defense and development. In this study, we identified a novel MYC2 TF from N. attenuata and characterized its regulatory function using a combination of molecular, analytic and ecological methods. RESULTS: The transcript and targeted metabolite analyses demonstrated that NaMYC2 is mainly involved in the regulation of the biosynthesis of nicotine and phenolamides in N. attenuata. In addition, using broadly-targeted metabolite analysis, we identified a number of other metabolite features that were regulated by NaMYC2, which, after full annotation, are expected to broaden our understanding of plant defense regulation. Unlike previous reports, the biosynthesis of jasmonates and some JA-/NaCOI1-dependent metabolites (e.g. HGL-DTGs) were not strongly regulated by NaMYC2, suggesting the involvement of other independent regulators. No significant differences were observed in the performance of M. sexta on MYC2-silenced plants, consistent with the well-known ability of this specialist insect to tolerate nicotine. CONCLUSION: By regulating the biosynthesis of nicotine, NaMYC2 is likely to enhance plant resistance against non-adapted herbivores and contribute to plant fitness; however, multiple JA/NaCOI1-dependent mechanisms (perhaps involving other MYCs) that regulate separate defense responses are likely to exist in N. attenuata. The considerable variation observed amongst different plant families in the responses regulated by jasmonate signaling highlights the sophistication with which plants craft highly specific and fine-tuned responses against the herbivores that attack them.
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spelling pubmed-36559062013-05-17 NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata Woldemariam, Melkamu G Dinh, Son Truong Oh, Youngjoo Gaquerel, Emmanuel Baldwin, Ian T Galis, Ivan BMC Plant Biol Research Article BACKGROUND: To survive herbivore attack, plants have evolved potent mechanisms of mechanical or chemical defense that are either constitutively present or inducible after herbivore attack. Due to the costs of defense deployment, plants often regulate their biosynthesis using various transcription factors (TFs). MYC2 regulators belong to the bHLH family of transcription factors that are involved in many aspects of plant defense and development. In this study, we identified a novel MYC2 TF from N. attenuata and characterized its regulatory function using a combination of molecular, analytic and ecological methods. RESULTS: The transcript and targeted metabolite analyses demonstrated that NaMYC2 is mainly involved in the regulation of the biosynthesis of nicotine and phenolamides in N. attenuata. In addition, using broadly-targeted metabolite analysis, we identified a number of other metabolite features that were regulated by NaMYC2, which, after full annotation, are expected to broaden our understanding of plant defense regulation. Unlike previous reports, the biosynthesis of jasmonates and some JA-/NaCOI1-dependent metabolites (e.g. HGL-DTGs) were not strongly regulated by NaMYC2, suggesting the involvement of other independent regulators. No significant differences were observed in the performance of M. sexta on MYC2-silenced plants, consistent with the well-known ability of this specialist insect to tolerate nicotine. CONCLUSION: By regulating the biosynthesis of nicotine, NaMYC2 is likely to enhance plant resistance against non-adapted herbivores and contribute to plant fitness; however, multiple JA/NaCOI1-dependent mechanisms (perhaps involving other MYCs) that regulate separate defense responses are likely to exist in N. attenuata. The considerable variation observed amongst different plant families in the responses regulated by jasmonate signaling highlights the sophistication with which plants craft highly specific and fine-tuned responses against the herbivores that attack them. BioMed Central 2013-05-01 /pmc/articles/PMC3655906/ /pubmed/23634896 http://dx.doi.org/10.1186/1471-2229-13-73 Text en Copyright © 2013 Woldemariam et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Woldemariam, Melkamu G
Dinh, Son Truong
Oh, Youngjoo
Gaquerel, Emmanuel
Baldwin, Ian T
Galis, Ivan
NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata
title NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata
title_full NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata
title_fullStr NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata
title_full_unstemmed NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata
title_short NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata
title_sort namyc2 transcription factor regulates a subset of plant defense responses in nicotiana attenuata
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655906/
https://www.ncbi.nlm.nih.gov/pubmed/23634896
http://dx.doi.org/10.1186/1471-2229-13-73
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