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Multi-Signal Sedimentation Velocity Analysis with Mass Conservation for Determining the Stoichiometry of Protein Complexes

Multi-signal sedimentation velocity analytical ultracentrifugation (MSSV) is a powerful tool for the determination of the number, stoichiometry, and hydrodynamic shape of reversible protein complexes in two- and three-component systems. In this method, the evolution of sedimentation profiles of macr...

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Detalles Bibliográficos
Autores principales: Brautigam, Chad A., Padrick, Shae B., Schuck, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656001/
https://www.ncbi.nlm.nih.gov/pubmed/23696787
http://dx.doi.org/10.1371/journal.pone.0062694