Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa

In Pseudomonas aeruginosa eDNA is a crucial component essential for biofilm formation and stability. In this study we report that release of eDNA is influenced by the production of phenazine in P. aeruginosa. A ∆phzA-G mutant of P. aeruginosa PA14 deficient in phenazine production generated signific...

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Detalles Bibliográficos
Autores principales: Das, Theerthankar, Manefield, Mike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656008/
https://www.ncbi.nlm.nih.gov/pubmed/23710274
http://dx.doi.org/10.4161/cib.23570
Descripción
Sumario:In Pseudomonas aeruginosa eDNA is a crucial component essential for biofilm formation and stability. In this study we report that release of eDNA is influenced by the production of phenazine in P. aeruginosa. A ∆phzA-G mutant of P. aeruginosa PA14 deficient in phenazine production generated significantly less eDNA in comparison with the phenazine producing strains. The relationship between eDNA release and phenazine production is bridged via hydrogen peroxide (H(2)O(2)) generation and subsequent H(2)O(2) mediated cell lysis and ultimately release of chromosomal DNA into the extracellular environment as eDNA.

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