Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa

In Pseudomonas aeruginosa eDNA is a crucial component essential for biofilm formation and stability. In this study we report that release of eDNA is influenced by the production of phenazine in P. aeruginosa. A ∆phzA-G mutant of P. aeruginosa PA14 deficient in phenazine production generated signific...

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Detalles Bibliográficos
Autores principales: Das, Theerthankar, Manefield, Mike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656008/
https://www.ncbi.nlm.nih.gov/pubmed/23710274
http://dx.doi.org/10.4161/cib.23570
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author Das, Theerthankar
Manefield, Mike
author_facet Das, Theerthankar
Manefield, Mike
author_sort Das, Theerthankar
collection PubMed
description In Pseudomonas aeruginosa eDNA is a crucial component essential for biofilm formation and stability. In this study we report that release of eDNA is influenced by the production of phenazine in P. aeruginosa. A ∆phzA-G mutant of P. aeruginosa PA14 deficient in phenazine production generated significantly less eDNA in comparison with the phenazine producing strains. The relationship between eDNA release and phenazine production is bridged via hydrogen peroxide (H(2)O(2)) generation and subsequent H(2)O(2) mediated cell lysis and ultimately release of chromosomal DNA into the extracellular environment as eDNA.
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spelling pubmed-36560082013-05-24 Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa Das, Theerthankar Manefield, Mike Commun Integr Biol Short Communication In Pseudomonas aeruginosa eDNA is a crucial component essential for biofilm formation and stability. In this study we report that release of eDNA is influenced by the production of phenazine in P. aeruginosa. A ∆phzA-G mutant of P. aeruginosa PA14 deficient in phenazine production generated significantly less eDNA in comparison with the phenazine producing strains. The relationship between eDNA release and phenazine production is bridged via hydrogen peroxide (H(2)O(2)) generation and subsequent H(2)O(2) mediated cell lysis and ultimately release of chromosomal DNA into the extracellular environment as eDNA. Landes Bioscience 2013-05-01 2013-05-01 /pmc/articles/PMC3656008/ /pubmed/23710274 http://dx.doi.org/10.4161/cib.23570 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Short Communication
Das, Theerthankar
Manefield, Mike
Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa
title Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa
title_full Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa
title_fullStr Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa
title_full_unstemmed Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa
title_short Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa
title_sort phenazine production enhances extracellular dna release via hydrogen peroxide generation in pseudomonas aeruginosa
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656008/
https://www.ncbi.nlm.nih.gov/pubmed/23710274
http://dx.doi.org/10.4161/cib.23570
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AT manefieldmike phenazineproductionenhancesextracellulardnareleaseviahydrogenperoxidegenerationinpseudomonasaeruginosa

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