Nuclear envelope-associated dynein cooperates with Eg5 to drive prophase centrosome separation

Eg5 (kinesin-5) is a highly conserved microtubule motor protein, essential for centrosome separation and bipolar spindle assembly in human cells. Using an “in vitro” evolution approach, we generated human cancer cells that can grow in the complete absence of Eg5 activity. Characterization of these E...

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Detalles Bibliográficos
Autores principales: van Heesbeen, Roy G.H.P., Raaijmakers, Jonne A., Tanenbaum, Marvin E., Medema, René H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Article Addendum
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656018/
https://www.ncbi.nlm.nih.gov/pubmed/23713137
http://dx.doi.org/10.4161/cib.23841
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author van Heesbeen, Roy G.H.P.
Raaijmakers, Jonne A.
Tanenbaum, Marvin E.
Medema, René H.
author_facet van Heesbeen, Roy G.H.P.
Raaijmakers, Jonne A.
Tanenbaum, Marvin E.
Medema, René H.
author_sort van Heesbeen, Roy G.H.P.
collection PubMed
description Eg5 (kinesin-5) is a highly conserved microtubule motor protein, essential for centrosome separation and bipolar spindle assembly in human cells. Using an “in vitro” evolution approach, we generated human cancer cells that can grow in the complete absence of Eg5 activity. Characterization of these Eg5-independent cells (EICs) led to the identification of a novel pathway for prophase centrosome separation, which depends on nuclear envelope (NE)-associated dynein. Here, we discuss our recent findings and elaborate on the mechanism by which dynein drives centrosome separation.
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spelling pubmed-36560182013-05-24 Nuclear envelope-associated dynein cooperates with Eg5 to drive prophase centrosome separation van Heesbeen, Roy G.H.P. Raaijmakers, Jonne A. Tanenbaum, Marvin E. Medema, René H. Commun Integr Biol Article Addendum Eg5 (kinesin-5) is a highly conserved microtubule motor protein, essential for centrosome separation and bipolar spindle assembly in human cells. Using an “in vitro” evolution approach, we generated human cancer cells that can grow in the complete absence of Eg5 activity. Characterization of these Eg5-independent cells (EICs) led to the identification of a novel pathway for prophase centrosome separation, which depends on nuclear envelope (NE)-associated dynein. Here, we discuss our recent findings and elaborate on the mechanism by which dynein drives centrosome separation. Landes Bioscience 2013-05-01 2013-05-01 /pmc/articles/PMC3656018/ /pubmed/23713137 http://dx.doi.org/10.4161/cib.23841 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Article Addendum
van Heesbeen, Roy G.H.P.
Raaijmakers, Jonne A.
Tanenbaum, Marvin E.
Medema, René H.
Nuclear envelope-associated dynein cooperates with Eg5 to drive prophase centrosome separation
title Nuclear envelope-associated dynein cooperates with Eg5 to drive prophase centrosome separation
title_full Nuclear envelope-associated dynein cooperates with Eg5 to drive prophase centrosome separation
title_fullStr Nuclear envelope-associated dynein cooperates with Eg5 to drive prophase centrosome separation
title_full_unstemmed Nuclear envelope-associated dynein cooperates with Eg5 to drive prophase centrosome separation
title_short Nuclear envelope-associated dynein cooperates with Eg5 to drive prophase centrosome separation
title_sort nuclear envelope-associated dynein cooperates with eg5 to drive prophase centrosome separation
topic Article Addendum
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656018/
https://www.ncbi.nlm.nih.gov/pubmed/23713137
http://dx.doi.org/10.4161/cib.23841
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