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Mass spectrometry imaging with high resolution in mass and space

Mass spectrometry (MS) imaging links molecular information and the spatial distribution of analytes within a sample. In contrast to most histochemical techniques, mass spectrometry imaging can differentiate molecular modifications and does not require labeling of targeted compounds. We have recently...

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Detalles Bibliográficos
Autores principales: Römpp, Andreas, Spengler, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656243/
https://www.ncbi.nlm.nih.gov/pubmed/23652571
http://dx.doi.org/10.1007/s00418-013-1097-6
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author Römpp, Andreas
Spengler, Bernhard
author_facet Römpp, Andreas
Spengler, Bernhard
author_sort Römpp, Andreas
collection PubMed
description Mass spectrometry (MS) imaging links molecular information and the spatial distribution of analytes within a sample. In contrast to most histochemical techniques, mass spectrometry imaging can differentiate molecular modifications and does not require labeling of targeted compounds. We have recently introduced the first mass spectrometry imaging method that provides highly specific molecular information (high resolution and accuracy in mass) at cellular dimensions (high resolution in space). This method is based on a matrix-assisted laser desorption/ionization (MALDI) imaging source working at atmospheric pressure which is coupled to an orbital trapping mass spectrometer. Here, we present a number of application examples and demonstrate the benefit of ‘mass spectrometry imaging with high resolution in mass and space.’ Phospholipids, peptides and drug compounds were imaged in a number of tissue samples at a spatial resolution of 5–10 μm. Proteins were analyzed after on-tissue tryptic digestion at 50-μm resolution. Additional applications include the analysis of single cells and of human lung carcinoma tissue as well as the first MALDI imaging measurement of tissue at 3 μm pixel size. MS image analysis for all these experiments showed excellent correlation with histological staining evaluation. The high mass resolution (R = 30,000) and mass accuracy (typically 1 ppm) proved to be essential for specific image generation and reliable identification of analytes in tissue samples. The ability to combine the required high-quality mass analysis with spatial resolution in the range of single cells is a unique feature of our method. With that, it has the potential to supplement classical histochemical protocols and to provide new insights about molecular processes on the cellular level.
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spelling pubmed-36562432013-05-17 Mass spectrometry imaging with high resolution in mass and space Römpp, Andreas Spengler, Bernhard Histochem Cell Biol Review Mass spectrometry (MS) imaging links molecular information and the spatial distribution of analytes within a sample. In contrast to most histochemical techniques, mass spectrometry imaging can differentiate molecular modifications and does not require labeling of targeted compounds. We have recently introduced the first mass spectrometry imaging method that provides highly specific molecular information (high resolution and accuracy in mass) at cellular dimensions (high resolution in space). This method is based on a matrix-assisted laser desorption/ionization (MALDI) imaging source working at atmospheric pressure which is coupled to an orbital trapping mass spectrometer. Here, we present a number of application examples and demonstrate the benefit of ‘mass spectrometry imaging with high resolution in mass and space.’ Phospholipids, peptides and drug compounds were imaged in a number of tissue samples at a spatial resolution of 5–10 μm. Proteins were analyzed after on-tissue tryptic digestion at 50-μm resolution. Additional applications include the analysis of single cells and of human lung carcinoma tissue as well as the first MALDI imaging measurement of tissue at 3 μm pixel size. MS image analysis for all these experiments showed excellent correlation with histological staining evaluation. The high mass resolution (R = 30,000) and mass accuracy (typically 1 ppm) proved to be essential for specific image generation and reliable identification of analytes in tissue samples. The ability to combine the required high-quality mass analysis with spatial resolution in the range of single cells is a unique feature of our method. With that, it has the potential to supplement classical histochemical protocols and to provide new insights about molecular processes on the cellular level. Springer-Verlag 2013-05-08 2013 /pmc/articles/PMC3656243/ /pubmed/23652571 http://dx.doi.org/10.1007/s00418-013-1097-6 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Review
Römpp, Andreas
Spengler, Bernhard
Mass spectrometry imaging with high resolution in mass and space
title Mass spectrometry imaging with high resolution in mass and space
title_full Mass spectrometry imaging with high resolution in mass and space
title_fullStr Mass spectrometry imaging with high resolution in mass and space
title_full_unstemmed Mass spectrometry imaging with high resolution in mass and space
title_short Mass spectrometry imaging with high resolution in mass and space
title_sort mass spectrometry imaging with high resolution in mass and space
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656243/
https://www.ncbi.nlm.nih.gov/pubmed/23652571
http://dx.doi.org/10.1007/s00418-013-1097-6
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