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Use of the tetrazolium salt MTT to measure cell viability effects of the bacterial antagonist Lysobacter enzymogenes on the filamentous fungus Cryphonectria parasitica
Despite substantial interest investigating bacterial mechanisms of fungal growth inhibition, there are few methods available that quantify fungal cell death during direct interactions with bacteria. Here we describe an in vitro cell suspension assay using the tetrazolium salt MTT as a viability stai...
Autores principales: | Patel, Nrupali, Oudemans, Peter V., Hillman, Bradley I., Kobayashi, Donald Y. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656244/ https://www.ncbi.nlm.nih.gov/pubmed/23529159 http://dx.doi.org/10.1007/s10482-013-9907-3 |
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