Overexpression of a MADS-Box Gene from Birch (Betula platyphylla) Promotes Flowering and Enhances Chloroplast Development in Transgenic Tobacco

In this study, a MADS-box gene (BpMADS), which is an ortholog of AP1 from Arabidopsis, was isolated from birch (Betula platyphylla). Transgenic Arabidopsis containing a BpMADS promoter::GUS construct was produced, which exhibited strong GUS staining in sepal tissues. Ectopic expression of BpMADS sig...

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Autores principales: Qu, Guan-Zheng, Zheng, Tangchun, Liu, Guifeng, Wang, Wenjie, Zang, Lina, Liu, Huanzhen, Yang, Chuanping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656909/
https://www.ncbi.nlm.nih.gov/pubmed/23691043
http://dx.doi.org/10.1371/journal.pone.0063398
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author Qu, Guan-Zheng
Zheng, Tangchun
Liu, Guifeng
Wang, Wenjie
Zang, Lina
Liu, Huanzhen
Yang, Chuanping
author_facet Qu, Guan-Zheng
Zheng, Tangchun
Liu, Guifeng
Wang, Wenjie
Zang, Lina
Liu, Huanzhen
Yang, Chuanping
author_sort Qu, Guan-Zheng
collection PubMed
description In this study, a MADS-box gene (BpMADS), which is an ortholog of AP1 from Arabidopsis, was isolated from birch (Betula platyphylla). Transgenic Arabidopsis containing a BpMADS promoter::GUS construct was produced, which exhibited strong GUS staining in sepal tissues. Ectopic expression of BpMADS significantly enhanced the flowering of tobacco (35S::BpMADS). In addition, the chloroplasts of transgenic tobacco exhibited much higher growth and division rates, as well rates of photosynthesis, than wild-type. A grafting experiment demonstrated that the flowering time of the scion was not affected by stock that overexpressed BpMADS. In addition, the overexpression of BpMADS resulted in the upregulation of some flowering-related genes in tobacco.
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spelling pubmed-36569092013-05-20 Overexpression of a MADS-Box Gene from Birch (Betula platyphylla) Promotes Flowering and Enhances Chloroplast Development in Transgenic Tobacco Qu, Guan-Zheng Zheng, Tangchun Liu, Guifeng Wang, Wenjie Zang, Lina Liu, Huanzhen Yang, Chuanping PLoS One Research Article In this study, a MADS-box gene (BpMADS), which is an ortholog of AP1 from Arabidopsis, was isolated from birch (Betula platyphylla). Transgenic Arabidopsis containing a BpMADS promoter::GUS construct was produced, which exhibited strong GUS staining in sepal tissues. Ectopic expression of BpMADS significantly enhanced the flowering of tobacco (35S::BpMADS). In addition, the chloroplasts of transgenic tobacco exhibited much higher growth and division rates, as well rates of photosynthesis, than wild-type. A grafting experiment demonstrated that the flowering time of the scion was not affected by stock that overexpressed BpMADS. In addition, the overexpression of BpMADS resulted in the upregulation of some flowering-related genes in tobacco. Public Library of Science 2013-05-17 /pmc/articles/PMC3656909/ /pubmed/23691043 http://dx.doi.org/10.1371/journal.pone.0063398 Text en © 2013 Qu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Qu, Guan-Zheng
Zheng, Tangchun
Liu, Guifeng
Wang, Wenjie
Zang, Lina
Liu, Huanzhen
Yang, Chuanping
Overexpression of a MADS-Box Gene from Birch (Betula platyphylla) Promotes Flowering and Enhances Chloroplast Development in Transgenic Tobacco
title Overexpression of a MADS-Box Gene from Birch (Betula platyphylla) Promotes Flowering and Enhances Chloroplast Development in Transgenic Tobacco
title_full Overexpression of a MADS-Box Gene from Birch (Betula platyphylla) Promotes Flowering and Enhances Chloroplast Development in Transgenic Tobacco
title_fullStr Overexpression of a MADS-Box Gene from Birch (Betula platyphylla) Promotes Flowering and Enhances Chloroplast Development in Transgenic Tobacco
title_full_unstemmed Overexpression of a MADS-Box Gene from Birch (Betula platyphylla) Promotes Flowering and Enhances Chloroplast Development in Transgenic Tobacco
title_short Overexpression of a MADS-Box Gene from Birch (Betula platyphylla) Promotes Flowering and Enhances Chloroplast Development in Transgenic Tobacco
title_sort overexpression of a mads-box gene from birch (betula platyphylla) promotes flowering and enhances chloroplast development in transgenic tobacco
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656909/
https://www.ncbi.nlm.nih.gov/pubmed/23691043
http://dx.doi.org/10.1371/journal.pone.0063398
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