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Directed evolution to re-adapt a co-evolved network within an enzyme

We have previously used targeted active-site saturation mutagenesis to identify a number of transketolase single mutants that improved activity towards either glycolaldehyde (GA), or the non-natural substrate propionaldehyde (PA). Here, all attempts to recombine the singles into double mutants led t...

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Detalles Bibliográficos
Autores principales:	Strafford, John, Payongsri, Panwajee, Hibbert, Edward G., Morris, Phattaraporn, Batth, Sukhjeet S., Steadman, David, Smith, Mark E.B., Ward, John M., Hailes, Helen C., Dalby, Paul A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Science Publishers 2012
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657141/ https://www.ncbi.nlm.nih.gov/pubmed/22154561 http://dx.doi.org/10.1016/j.jbiotec.2011.11.017

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