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An enzyme-linked immunosorbent assay (ELISA) for quantification of human collectin 11 (CL-11, CL-K1)
Collectin 11 (CL-11), also referred to as collectin kidney 1 (CL-K1), is a pattern recognition molecule that belongs to the collectin group of proteins involved in innate immunity. It interacts with glycoconjugates on pathogen surfaces and has been found in complex with mannose-binding lectin-associ...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657160/ https://www.ncbi.nlm.nih.gov/pubmed/22301270 http://dx.doi.org/10.1016/j.jim.2011.10.010 |
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author | Selman, L. Henriksen, M.L. Brandt, J. Palarasah, Y. Waters, A. Beales, P.L. Holmskov, U. Jørgensen, T.J.D. Nielsen, C. Skjodt, K. Hansen, S. |
author_facet | Selman, L. Henriksen, M.L. Brandt, J. Palarasah, Y. Waters, A. Beales, P.L. Holmskov, U. Jørgensen, T.J.D. Nielsen, C. Skjodt, K. Hansen, S. |
author_sort | Selman, L. |
collection | PubMed |
description | Collectin 11 (CL-11), also referred to as collectin kidney 1 (CL-K1), is a pattern recognition molecule that belongs to the collectin group of proteins involved in innate immunity. It interacts with glycoconjugates on pathogen surfaces and has been found in complex with mannose-binding lectin-associated serine protease 1 (MASP-1) and/or MASP-3 in circulation. Mutation in the CL-11 gene was recently associated with the developmental syndrome 3MC. In the present study, we established and thoroughly validated a sandwich enzyme-linked immunosorbent assay (ELISA) based on two different monoclonal antibodies. The assay is highly sensitive, specific and shows excellent quantitative characteristics such as reproducibility, dilution linearity and recovery (97.7–104%). The working range is 0.15–34 ng/ml. The CL-11 concentration in two CL-11-deficient individuals affected by the 3MC syndrome was determined to be below 2.1 ng/ml. We measured the mean serum CL-11 concentration to 284 ng/ml in 100 Danish blood donors, with a 95% confidence interval of 269–299 ng/ml. There was no significant difference in the CL-11 concentration measured in matched serum and plasma samples. Storage of samples and repeated freezing and thawing to a certain extent did not influence the ELISA. This ELISA offers a convenient and reliable method for studying CL-11 levels in relation to a variety of human diseases and syndromes. |
format | Online Article Text |
id | pubmed-3657160 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-36571602013-05-18 An enzyme-linked immunosorbent assay (ELISA) for quantification of human collectin 11 (CL-11, CL-K1) Selman, L. Henriksen, M.L. Brandt, J. Palarasah, Y. Waters, A. Beales, P.L. Holmskov, U. Jørgensen, T.J.D. Nielsen, C. Skjodt, K. Hansen, S. J Immunol Methods Research Paper Collectin 11 (CL-11), also referred to as collectin kidney 1 (CL-K1), is a pattern recognition molecule that belongs to the collectin group of proteins involved in innate immunity. It interacts with glycoconjugates on pathogen surfaces and has been found in complex with mannose-binding lectin-associated serine protease 1 (MASP-1) and/or MASP-3 in circulation. Mutation in the CL-11 gene was recently associated with the developmental syndrome 3MC. In the present study, we established and thoroughly validated a sandwich enzyme-linked immunosorbent assay (ELISA) based on two different monoclonal antibodies. The assay is highly sensitive, specific and shows excellent quantitative characteristics such as reproducibility, dilution linearity and recovery (97.7–104%). The working range is 0.15–34 ng/ml. The CL-11 concentration in two CL-11-deficient individuals affected by the 3MC syndrome was determined to be below 2.1 ng/ml. We measured the mean serum CL-11 concentration to 284 ng/ml in 100 Danish blood donors, with a 95% confidence interval of 269–299 ng/ml. There was no significant difference in the CL-11 concentration measured in matched serum and plasma samples. Storage of samples and repeated freezing and thawing to a certain extent did not influence the ELISA. This ELISA offers a convenient and reliable method for studying CL-11 levels in relation to a variety of human diseases and syndromes. Elsevier 2012-01-31 /pmc/articles/PMC3657160/ /pubmed/22301270 http://dx.doi.org/10.1016/j.jim.2011.10.010 Text en © 2012 Elsevier B.V. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Research Paper Selman, L. Henriksen, M.L. Brandt, J. Palarasah, Y. Waters, A. Beales, P.L. Holmskov, U. Jørgensen, T.J.D. Nielsen, C. Skjodt, K. Hansen, S. An enzyme-linked immunosorbent assay (ELISA) for quantification of human collectin 11 (CL-11, CL-K1) |
title | An enzyme-linked immunosorbent assay (ELISA) for quantification of human collectin 11 (CL-11, CL-K1) |
title_full | An enzyme-linked immunosorbent assay (ELISA) for quantification of human collectin 11 (CL-11, CL-K1) |
title_fullStr | An enzyme-linked immunosorbent assay (ELISA) for quantification of human collectin 11 (CL-11, CL-K1) |
title_full_unstemmed | An enzyme-linked immunosorbent assay (ELISA) for quantification of human collectin 11 (CL-11, CL-K1) |
title_short | An enzyme-linked immunosorbent assay (ELISA) for quantification of human collectin 11 (CL-11, CL-K1) |
title_sort | enzyme-linked immunosorbent assay (elisa) for quantification of human collectin 11 (cl-11, cl-k1) |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657160/ https://www.ncbi.nlm.nih.gov/pubmed/22301270 http://dx.doi.org/10.1016/j.jim.2011.10.010 |
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