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Host gene expression changes in cattle infected with Alcelaphine herpesvirus 1

Malignant catarrhal fever is a lymphoproliferative disease of cattle and other ungulates caused by infection with gamma-herpesviruses of the genus Macavirus. These viruses do not establish a productive infection but instead replicate in a cell-associated fashion in T lymphocytes, leading to systemic...

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Autores principales: Russell, George C., Benavides, Julio, Grant, Dawn M., Todd, Helen, Thomson, Jackie, Puri, Vipul, Nath, Mintu, Haig, David M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657188/
https://www.ncbi.nlm.nih.gov/pubmed/22925730
http://dx.doi.org/10.1016/j.virusres.2012.08.011
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author Russell, George C.
Benavides, Julio
Grant, Dawn M.
Todd, Helen
Thomson, Jackie
Puri, Vipul
Nath, Mintu
Haig, David M.
author_facet Russell, George C.
Benavides, Julio
Grant, Dawn M.
Todd, Helen
Thomson, Jackie
Puri, Vipul
Nath, Mintu
Haig, David M.
author_sort Russell, George C.
collection PubMed
description Malignant catarrhal fever is a lymphoproliferative disease of cattle and other ungulates caused by infection with gamma-herpesviruses of the genus Macavirus. These viruses do not establish a productive infection but instead replicate in a cell-associated fashion in T lymphocytes, leading to systemic immune dysregulation and a generally fatal outcome. Despite significant progress in understanding the pathology of this disease, its pathogenesis remains unclear. To identify genes and pathways affected in clinical MCF, sixteen bovine GeneCHIP microarrays were used to assay RNA from kidney and lymph node of four MCF-affected and four control Bos taurus steers. This is the first expression study of AlHV-1-MCF in the bovine host. Over 250 genes showed significant changes in gene expression in either lymph node or kidney, while expression of 35 genes was altered in both tissues. Pathway and annotation analysis of the microarray data showed that immune response and inflammatory genes were up-regulated in the kidney while proliferation-associated transcripts were additionally increased in the lymph node. The genes that showed the largest expression rises in both diseased tissues included cytotoxic enzymes and pro-inflammatory chemokines. These data are consistent with disease-induced stimulation of inflammatory responses involving interferon-γ, including cytotoxic T cell recruitment and activation in peripheral tissues containing virus-infected cells. However it remains unclear whether the tissue damage in MCF lesions is due entirely to the activity of infected cells or whether uninfected T cells, recruited and activated at lesion sites through the action of infected cells, contribute to the pathogenesis of MCF.
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spelling pubmed-36571882013-05-18 Host gene expression changes in cattle infected with Alcelaphine herpesvirus 1 Russell, George C. Benavides, Julio Grant, Dawn M. Todd, Helen Thomson, Jackie Puri, Vipul Nath, Mintu Haig, David M. Virus Res Article Malignant catarrhal fever is a lymphoproliferative disease of cattle and other ungulates caused by infection with gamma-herpesviruses of the genus Macavirus. These viruses do not establish a productive infection but instead replicate in a cell-associated fashion in T lymphocytes, leading to systemic immune dysregulation and a generally fatal outcome. Despite significant progress in understanding the pathology of this disease, its pathogenesis remains unclear. To identify genes and pathways affected in clinical MCF, sixteen bovine GeneCHIP microarrays were used to assay RNA from kidney and lymph node of four MCF-affected and four control Bos taurus steers. This is the first expression study of AlHV-1-MCF in the bovine host. Over 250 genes showed significant changes in gene expression in either lymph node or kidney, while expression of 35 genes was altered in both tissues. Pathway and annotation analysis of the microarray data showed that immune response and inflammatory genes were up-regulated in the kidney while proliferation-associated transcripts were additionally increased in the lymph node. The genes that showed the largest expression rises in both diseased tissues included cytotoxic enzymes and pro-inflammatory chemokines. These data are consistent with disease-induced stimulation of inflammatory responses involving interferon-γ, including cytotoxic T cell recruitment and activation in peripheral tissues containing virus-infected cells. However it remains unclear whether the tissue damage in MCF lesions is due entirely to the activity of infected cells or whether uninfected T cells, recruited and activated at lesion sites through the action of infected cells, contribute to the pathogenesis of MCF. Elsevier Science 2012-10 /pmc/articles/PMC3657188/ /pubmed/22925730 http://dx.doi.org/10.1016/j.virusres.2012.08.011 Text en © 2012 Elsevier B.V. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Russell, George C.
Benavides, Julio
Grant, Dawn M.
Todd, Helen
Thomson, Jackie
Puri, Vipul
Nath, Mintu
Haig, David M.
Host gene expression changes in cattle infected with Alcelaphine herpesvirus 1
title Host gene expression changes in cattle infected with Alcelaphine herpesvirus 1
title_full Host gene expression changes in cattle infected with Alcelaphine herpesvirus 1
title_fullStr Host gene expression changes in cattle infected with Alcelaphine herpesvirus 1
title_full_unstemmed Host gene expression changes in cattle infected with Alcelaphine herpesvirus 1
title_short Host gene expression changes in cattle infected with Alcelaphine herpesvirus 1
title_sort host gene expression changes in cattle infected with alcelaphine herpesvirus 1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657188/
https://www.ncbi.nlm.nih.gov/pubmed/22925730
http://dx.doi.org/10.1016/j.virusres.2012.08.011
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