Cargando…

2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice

Consumption of isoflavones may prevent adiposity, hepatic steatosis, and dyslipidaemia. However, studies in the area are few and primarily with genistein. This study investigated the effects of formononetin and its synthetic analogue, 2-heptyl-formononetin (C7F), on lipid and cholesterol metabolism...

Descripción completa

Detalles Bibliográficos
Autores principales: Andersen, Charlotte, Schjoldager, Janne G., Tortzen, Christian G., Vegge, Andreas, Hufeldt, Majbritt R., Skaanild, Mette T., Vogensen, Finn K., Kristiansen, Karsten, Hansen, Axel K., Nielsen, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657407/
https://www.ncbi.nlm.nih.gov/pubmed/23738334
http://dx.doi.org/10.1155/2013/926942
_version_ 1782270133055520768
author Andersen, Charlotte
Schjoldager, Janne G.
Tortzen, Christian G.
Vegge, Andreas
Hufeldt, Majbritt R.
Skaanild, Mette T.
Vogensen, Finn K.
Kristiansen, Karsten
Hansen, Axel K.
Nielsen, John
author_facet Andersen, Charlotte
Schjoldager, Janne G.
Tortzen, Christian G.
Vegge, Andreas
Hufeldt, Majbritt R.
Skaanild, Mette T.
Vogensen, Finn K.
Kristiansen, Karsten
Hansen, Axel K.
Nielsen, John
author_sort Andersen, Charlotte
collection PubMed
description Consumption of isoflavones may prevent adiposity, hepatic steatosis, and dyslipidaemia. However, studies in the area are few and primarily with genistein. This study investigated the effects of formononetin and its synthetic analogue, 2-heptyl-formononetin (C7F), on lipid and cholesterol metabolism in C57BL/6J mice. The mice were fed a cholesterol-enriched diet for five weeks to induce hypercholesterolemia and were then fed either the cholesterol-enriched diet or the cholesterol-enriched diet-supplemented formononetin or C7F for three weeks. Body weight and composition, glucose homeostasis, and plasma lipids were compared. In another experiment, mice were fed the above diets for five weeks, and hepatic triglyceride accumulation and gene expression and histology of adipose tissue and liver were examined. Supplementation with C7F increased plasma HDL-cholesterol thereby increasing the plasma level of total cholesterol. Supplementation with formononetin did not affect plasma cholesterol but increased plasma triglycerides levels. Supplementation with formononetin and C7F induced hepatic steatosis. However, formononetin decreased markers of inflammation and liver injury. The development of hepatic steatosis was associated with deregulated expression of hepatic genes involved in lipid and lipoprotein metabolism. In conclusion, supplementation with formononetin and C7F to a cholesterol-enriched diet adversely affected lipid and lipoprotein metabolism in C57BL/6J mice.
format Online
Article
Text
id pubmed-3657407
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-36574072013-06-04 2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice Andersen, Charlotte Schjoldager, Janne G. Tortzen, Christian G. Vegge, Andreas Hufeldt, Majbritt R. Skaanild, Mette T. Vogensen, Finn K. Kristiansen, Karsten Hansen, Axel K. Nielsen, John Biomed Res Int Research Article Consumption of isoflavones may prevent adiposity, hepatic steatosis, and dyslipidaemia. However, studies in the area are few and primarily with genistein. This study investigated the effects of formononetin and its synthetic analogue, 2-heptyl-formononetin (C7F), on lipid and cholesterol metabolism in C57BL/6J mice. The mice were fed a cholesterol-enriched diet for five weeks to induce hypercholesterolemia and were then fed either the cholesterol-enriched diet or the cholesterol-enriched diet-supplemented formononetin or C7F for three weeks. Body weight and composition, glucose homeostasis, and plasma lipids were compared. In another experiment, mice were fed the above diets for five weeks, and hepatic triglyceride accumulation and gene expression and histology of adipose tissue and liver were examined. Supplementation with C7F increased plasma HDL-cholesterol thereby increasing the plasma level of total cholesterol. Supplementation with formononetin did not affect plasma cholesterol but increased plasma triglycerides levels. Supplementation with formononetin and C7F induced hepatic steatosis. However, formononetin decreased markers of inflammation and liver injury. The development of hepatic steatosis was associated with deregulated expression of hepatic genes involved in lipid and lipoprotein metabolism. In conclusion, supplementation with formononetin and C7F to a cholesterol-enriched diet adversely affected lipid and lipoprotein metabolism in C57BL/6J mice. Hindawi Publishing Corporation 2013 2013-04-29 /pmc/articles/PMC3657407/ /pubmed/23738334 http://dx.doi.org/10.1155/2013/926942 Text en Copyright © 2013 Charlotte Andersen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Andersen, Charlotte
Schjoldager, Janne G.
Tortzen, Christian G.
Vegge, Andreas
Hufeldt, Majbritt R.
Skaanild, Mette T.
Vogensen, Finn K.
Kristiansen, Karsten
Hansen, Axel K.
Nielsen, John
2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice
title 2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice
title_full 2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice
title_fullStr 2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice
title_full_unstemmed 2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice
title_short 2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice
title_sort 2-heptyl-formononetin increases cholesterol and induces hepatic steatosis in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657407/
https://www.ncbi.nlm.nih.gov/pubmed/23738334
http://dx.doi.org/10.1155/2013/926942
work_keys_str_mv AT andersencharlotte 2heptylformononetinincreasescholesterolandinduceshepaticsteatosisinmice
AT schjoldagerjanneg 2heptylformononetinincreasescholesterolandinduceshepaticsteatosisinmice
AT tortzenchristiang 2heptylformononetinincreasescholesterolandinduceshepaticsteatosisinmice
AT veggeandreas 2heptylformononetinincreasescholesterolandinduceshepaticsteatosisinmice
AT hufeldtmajbrittr 2heptylformononetinincreasescholesterolandinduceshepaticsteatosisinmice
AT skaanildmettet 2heptylformononetinincreasescholesterolandinduceshepaticsteatosisinmice
AT vogensenfinnk 2heptylformononetinincreasescholesterolandinduceshepaticsteatosisinmice
AT kristiansenkarsten 2heptylformononetinincreasescholesterolandinduceshepaticsteatosisinmice
AT hansenaxelk 2heptylformononetinincreasescholesterolandinduceshepaticsteatosisinmice
AT nielsenjohn 2heptylformononetinincreasescholesterolandinduceshepaticsteatosisinmice