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How many neurons can we see with current spike sorting algorithms?

Recent studies highlighted the disagreement between the typical number of neurons observed with extracellular recordings and the ones to be expected based on anatomical and physiological considerations. This disagreement has been mainly attributed to the presence of sparsely firing neurons. However,...

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Detalles Bibliográficos
Autores principales: Pedreira, Carlos, Martinez, Juan, Ison, Matias J., Quian Quiroga, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier/North-Holland Biomedical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657693/
https://www.ncbi.nlm.nih.gov/pubmed/22841630
http://dx.doi.org/10.1016/j.jneumeth.2012.07.010
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author Pedreira, Carlos
Martinez, Juan
Ison, Matias J.
Quian Quiroga, Rodrigo
author_facet Pedreira, Carlos
Martinez, Juan
Ison, Matias J.
Quian Quiroga, Rodrigo
author_sort Pedreira, Carlos
collection PubMed
description Recent studies highlighted the disagreement between the typical number of neurons observed with extracellular recordings and the ones to be expected based on anatomical and physiological considerations. This disagreement has been mainly attributed to the presence of sparsely firing neurons. However, it is also possible that this is due to limitations of the spike sorting algorithms used to process the data. To address this issue, we used realistic simulations of extracellular recordings and found a relatively poor spike sorting performance for simulations containing a large number of neurons. In fact, the number of correctly identified neurons for single-channel recordings showed an asymptotic behavior saturating at about 8–10 units, when up to 20 units were present in the data. This performance was significantly poorer for neurons with low firing rates, as these units were twice more likely to be missed than the ones with high firing rates in simulations containing many neurons. These results uncover one of the main reasons for the relatively low number of neurons found in extracellular recording and also stress the importance of further developments of spike sorting algorithms.
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spelling pubmed-36576932013-05-20 How many neurons can we see with current spike sorting algorithms? Pedreira, Carlos Martinez, Juan Ison, Matias J. Quian Quiroga, Rodrigo J Neurosci Methods Computational Neuroscience Recent studies highlighted the disagreement between the typical number of neurons observed with extracellular recordings and the ones to be expected based on anatomical and physiological considerations. This disagreement has been mainly attributed to the presence of sparsely firing neurons. However, it is also possible that this is due to limitations of the spike sorting algorithms used to process the data. To address this issue, we used realistic simulations of extracellular recordings and found a relatively poor spike sorting performance for simulations containing a large number of neurons. In fact, the number of correctly identified neurons for single-channel recordings showed an asymptotic behavior saturating at about 8–10 units, when up to 20 units were present in the data. This performance was significantly poorer for neurons with low firing rates, as these units were twice more likely to be missed than the ones with high firing rates in simulations containing many neurons. These results uncover one of the main reasons for the relatively low number of neurons found in extracellular recording and also stress the importance of further developments of spike sorting algorithms. Elsevier/North-Holland Biomedical Press 2012-10-15 /pmc/articles/PMC3657693/ /pubmed/22841630 http://dx.doi.org/10.1016/j.jneumeth.2012.07.010 Text en © 2012 Elsevier B.V. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Computational Neuroscience
Pedreira, Carlos
Martinez, Juan
Ison, Matias J.
Quian Quiroga, Rodrigo
How many neurons can we see with current spike sorting algorithms?
title How many neurons can we see with current spike sorting algorithms?
title_full How many neurons can we see with current spike sorting algorithms?
title_fullStr How many neurons can we see with current spike sorting algorithms?
title_full_unstemmed How many neurons can we see with current spike sorting algorithms?
title_short How many neurons can we see with current spike sorting algorithms?
title_sort how many neurons can we see with current spike sorting algorithms?
topic Computational Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657693/
https://www.ncbi.nlm.nih.gov/pubmed/22841630
http://dx.doi.org/10.1016/j.jneumeth.2012.07.010
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