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Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle
Current approaches to monitor and quantify cell division in live cells, and reliably distinguish between acytokinesis and endoreduplication, are limited and complicate determination of stem cell pool identities. Here we overcome these limitations by generating an in vivo reporter system using the sc...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658003/ https://www.ncbi.nlm.nih.gov/pubmed/23011130 http://dx.doi.org/10.1038/ncomms2089 |
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author | Hesse, Michael Raulf, Alexandra Pilz, Gregor-Alexander Haberlandt, Christian Klein, Alexandra M. Jabs, Ronald Zaehres, Holm Fügemann, Christopher J. Zimmermann, Katrin Trebicka, Jonel Welz, Armin Pfeifer, Alexander Röll, Wilhelm Kotlikoff, Michael I. Steinhäuser, Christian Götz, Magdalena Schöler, Hans R. Fleischmann, Bernd K. |
author_facet | Hesse, Michael Raulf, Alexandra Pilz, Gregor-Alexander Haberlandt, Christian Klein, Alexandra M. Jabs, Ronald Zaehres, Holm Fügemann, Christopher J. Zimmermann, Katrin Trebicka, Jonel Welz, Armin Pfeifer, Alexander Röll, Wilhelm Kotlikoff, Michael I. Steinhäuser, Christian Götz, Magdalena Schöler, Hans R. Fleischmann, Bernd K. |
author_sort | Hesse, Michael |
collection | PubMed |
description | Current approaches to monitor and quantify cell division in live cells, and reliably distinguish between acytokinesis and endoreduplication, are limited and complicate determination of stem cell pool identities. Here we overcome these limitations by generating an in vivo reporter system using the scaffolding protein anillin fused to enhanced green fluorescent protein, to provide high spatiotemporal resolution of mitotic phase. This approach visualizes cytokinesis and midbody formation as hallmarks of expansion of stem and somatic cells, and enables distinction from cell cycle variations. High-resolution microscopy in embryonic heart and brain tissues of enhanced green fluorescent protein–anillin transgenic mice allows live monitoring of cell division and quantitation of cell cycle kinetics. Analysis of cell division in hearts post injury shows that border zone cardiomyocytes in the infarct respond with increasing ploidy, but not cell division. Thus, the enhanced green fluorescent protein–anillin system enables monitoring and measurement of cell division in vivo and markedly simplifies in vitro analysis in fixed cells. |
format | Online Article Text |
id | pubmed-3658003 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-36580032013-05-20 Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle Hesse, Michael Raulf, Alexandra Pilz, Gregor-Alexander Haberlandt, Christian Klein, Alexandra M. Jabs, Ronald Zaehres, Holm Fügemann, Christopher J. Zimmermann, Katrin Trebicka, Jonel Welz, Armin Pfeifer, Alexander Röll, Wilhelm Kotlikoff, Michael I. Steinhäuser, Christian Götz, Magdalena Schöler, Hans R. Fleischmann, Bernd K. Nat Commun Article Current approaches to monitor and quantify cell division in live cells, and reliably distinguish between acytokinesis and endoreduplication, are limited and complicate determination of stem cell pool identities. Here we overcome these limitations by generating an in vivo reporter system using the scaffolding protein anillin fused to enhanced green fluorescent protein, to provide high spatiotemporal resolution of mitotic phase. This approach visualizes cytokinesis and midbody formation as hallmarks of expansion of stem and somatic cells, and enables distinction from cell cycle variations. High-resolution microscopy in embryonic heart and brain tissues of enhanced green fluorescent protein–anillin transgenic mice allows live monitoring of cell division and quantitation of cell cycle kinetics. Analysis of cell division in hearts post injury shows that border zone cardiomyocytes in the infarct respond with increasing ploidy, but not cell division. Thus, the enhanced green fluorescent protein–anillin system enables monitoring and measurement of cell division in vivo and markedly simplifies in vitro analysis in fixed cells. Nature Pub. Group 2012-09-25 /pmc/articles/PMC3658003/ /pubmed/23011130 http://dx.doi.org/10.1038/ncomms2089 Text en Copyright © 2012, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
spellingShingle | Article Hesse, Michael Raulf, Alexandra Pilz, Gregor-Alexander Haberlandt, Christian Klein, Alexandra M. Jabs, Ronald Zaehres, Holm Fügemann, Christopher J. Zimmermann, Katrin Trebicka, Jonel Welz, Armin Pfeifer, Alexander Röll, Wilhelm Kotlikoff, Michael I. Steinhäuser, Christian Götz, Magdalena Schöler, Hans R. Fleischmann, Bernd K. Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle |
title | Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle |
title_full | Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle |
title_fullStr | Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle |
title_full_unstemmed | Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle |
title_short | Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle |
title_sort | direct visualization of cell division using high-resolution imaging of m-phase of the cell cycle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658003/ https://www.ncbi.nlm.nih.gov/pubmed/23011130 http://dx.doi.org/10.1038/ncomms2089 |
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