Sensitive and accurate estimation of losartan potassium formulation by high-performance thin-layer chromatography

AIM AND OBJECTIVES: To develop a simple, sensitive, cost-effective and reproducible UV-spectrophotometric method and validate for the estimation of disodium edetate in topical gel formulations. MATERIALS AND METHODS: Solution of disodium edetate reacts with ferric chloride to form complex in 0.1 N HCl giving λ(max) at 270 nm. Beer's law was obeyed in the concentration range of 5–50 μg/mL (r(2) = 0.9997). RESULTS: The limit of detection and limit of quantitation were found to be 1.190 and 3.608 μg/mL, respectively. The results show that the procedure is accurate, precise, and reproducible (relative standard deviation < 1%), while being simple and less time consuming. CONCLUSIONS: The study concluded that the UV-spectrophotometric method could be used for the quantification of disodium edetate in pure form as well as in pharmaceutical formulations.